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Anti-human complement C3 goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-human complement C3 goat IgG fraction, fluorescein-conjugated, 2 mL

Packaging size: 2 mL

SKU 0855167
$336.00
In Stock
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Description

Product Description

Fluorescein-conjugated Goat IgG Fraction to Human Complement C3 is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human complement C3 and buffer salts.

Application Notes

Fluorescein-conjugated Goat IgG Fraction to Human Complement C3 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). It is also used for Immunohistochemistry techniques at a dilution of 1:50.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855167
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-conjugated Goat IgG Fraction to Human Complement C3 is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). It is also used for Immunohistochemistry techniques at a dilution of 1:50.
Base Catalog Number 55167
Concentration Total Protein: 35.9 mg/mL; Antibody Protein: 7 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Human C3 purified from serum
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human, IgG Fraction
Specificity This product shows reactivity to human complement C3; cross-reactivity to other species may exist. Antibody activity to complement components C4-C9 or serum proteins is not present.
Typical Working Concentration Dilution Recommendations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.