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Anti-human IgM (5Fc µ) goat F(Ab')2 fragment, 2 mL

Anti-human IgM (5Fc µ) goat F(Ab')2 fragment, 2 mL

Packaging size: 2 mL

SKU 0855055
$244.50
In Stock
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Description

Product Description

Product is the lyophilized powder of goat F(ab')2 fragment to human IgM (5Fc μ) and buffer salts.

Application Notes

Goat F(AB')2 fragment to Human IgM is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855055
Antibody Type Polyclonal Antibody
Application Notes Goat F(AB')2 fragment to Human IgM is use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. (Note: Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Base Catalog Number 55055
Concentration Total Protein: 6.2 mg/mL; Antibody Protein: 3.5 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Goat
Pack Size 2 mL
Preparation Method The F(ab')<sub>2</sub> fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin, and purified by gel filtration or affinity chromatography. The resulting F(ab')<sub>2</sub> fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Purity F(ab')<sub>2</sub> fragment
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human, IgM (5Fc µ)
Specificity Product shows reactivity to human IgM; cross-reactivity to other species may exist. Antibody activity to human IgG; IgA; Fab or non-immunoglobulin serum proteins is not present.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.