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IgG2B (Kappa), mouse ascites, 5 mg
Product is the lyophilized powder of clarified mouse myeloma ascites IgG2b (j) MOPC 195.
This product is suitable for use as a positive control in antibody identification screening techniques. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Purified proteins are recommended as ELISA coating antigens and as controls.
Immunoassays | Immunoblot| Immunohistochemistry
|Alternate Names||Mouse IgG2b/k|
|Antibody Type||Monoclonal Antibody|
|Application Notes||This product is suitable for use as a positive control in antibody identification screening techniques. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. Purified proteins are recommended as ELISA coating antigens and as controls.|
|Base Catalog Number||50344|
|Clone Name||MOPC 195|
|Pack Size||5 mg|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|Preparation Method||The IgG2b (j) MOPC 195 tumor line is a mineral oil (Bayol F)-induced plasmacytoma. The clarified ascites is produced in BALB/c mice, and is filtered through a 0.22 µm filter, vialed and lyophilized|
|Solubility||Reconstitution: Reconstitute product with 1 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.|
|Specificity||The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Each ascites is tested for immunoglobulin content by radial immunodiffusion with specific antisera following precipitation by ammonium sulfate (See Protocol for Immunoglobulin Precipitation). In addition; the ascites contains the normal threshold of mouse immunoglobulins and ascites proteins.|
|Sterility||Filtered through a .22 µm filter|