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IgG3 (Kappa) FLOPC21, mouse myeloma, purified, 1 mg
These myeloma proteins are purified from ascites by a combination of salt precipitation, ion exchange and bioaffinity chromatography. Purified proteins are dialyzed into 0.02M Tris, 0.14M sodium chloride, pH 8.1. Product is adjusted to 1.0 mg/ml, filtered, vialed, and stored frozen at -70°C. Product is shipped on dry ice. Mouse IgG3 (k) is purified from clarified mouse FLOPC 21 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.
This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
|Antibody Type||Monoclonal Antibody|
|Application Notes||This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.|
|Base Catalog Number||50332|
|Clone Name||FLOPC 21|
|Concentration||Total Protein: 1.0 mg/mL; Volume: 1.0 mL|
|Pack Size||1 mg|
|Solubility||Soluble in Water|
|Specificity||The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG3. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG3; no trace of IgG1; IgG2a; IgG2b; IgA; IgM or non-immunoglobulin serum proteins is detected.|