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IgG2B (Kappa) MOPC 141, mouse myeloma, purified, 1 mg

IgG2B (Kappa) MOPC 141, mouse myeloma, purified, 1 mg

$222.65
Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02 M Tris 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

SKU: 0850331

Packaging size:
1 mg
Product Description

Purified mouse myeloma protein IgG2B (Kappa) is purified mouse IgG2b(j) and buffer. Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Positive control in antibody identification screening techniques

Specifications
SKU 0850331
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number 50331
Clone Name MOPC 141
Concentration Total Protein: 1.0 mg/mL; Volume: 1.0 mL
Conjugate Unconjugated
Formulation Details Purified
Host Mouse
Isotype IgG
Pack Size 1 mg
Preparation Method Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 μm filter and vialed. No preservative has been added.
Purity Affinity Purified
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2b. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2b; no trace of IgG1; IgG2a; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.