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Yeast lytic enzyme
Yeast Lytic Enzyme
Yeast Lytic Enzyme is used for cell lysis, spheroplasting and glucan hydrolysis.
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Cell Lysis; Protoplasm preparation
|Application Notes||Yeast Lytic Enzyme is used for cell lysis, spheroplasting and glucan hydrolysis.|
|Base Catalog Number||190123|
|Biochemical Physiological Actions||Primary enzyme in this preparation responsible for lysis of viable yeast cells is ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.|
|Specific Activity||≥100,000 u/g|
|Specificity||The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwanniomyces and others.|
|Unit Definition||One unit will produce a change in absorbance at 800 nm of 0.001 per minute at pH 7.5 and 25 °C, using a suspension of brewers yeast as a substrate in a 3 mL reaction mixture.|