UltraFi-Taq™ DNA Polymerase, 400 reactions

UltraFi-Taq™ DNA Polymerase is a high-fidelity DNA polymerase with the lowest error rate in the market. It is over 1000x more accurate than wild-type Taq DNA polymerase and offers unparalleled fidelity and specificity. This enzyme has been specifically engineered for hot-start PCR reactions by incorporating an antibody that blocks polymerase activity at low temperatures. This allows for reaction set-up at room temperature without the risk of non-specific amplifications and/or primer-dimer formation. At high temperatures, the antibody dissociates from the DNA polymerase, thus activating enzyme function and providing high specificity and sensitivity for robust, high-fidelity PCR amplifications

UltraFi-Taq DNA Polymerase displays increased processivity compared to wild-type Taq DNA polymerase, leading to higher product yields in less time. This enzyme is ideal for long range PCR amplifications up to 15 kb, next-generation DNA sequencing, mutagenesis, and confirmation of CRISPR-Cas editing.

Application Notes

UltraFi-Taq DNA Polymerase is 1000x more accurate than wild-type Taq DNA polymerase, providing unparalleled fidelity and specificity for use in key molecular biology applications, such as high-fidelity PCR, qPCR, RT-PCR, NGS, amplification of difficult targets, cDNA libraries, cloning, SNP analysis, CRISPR-Cas mutagenesis, etc.

Usage Statement

Research Use Only (RUO)

Key Applications

High-fidelity PCR; qPCR; long-range PCR; NGS; cDNA cloning; SNP analysis; mutagenesis; confirmation of CRISPR-Cas editing