SPINeasy® DNA/RNA/Protein All-In-One Kit, 50 preps
SKU: 116544050
SPINeasy DNA/RNA/Protein All-In-One Kit utilizes a convenient workflow and silica membrane spin columns to isolate DNA, RNA, and protein components from the same sample, without the use of toxic substances such as phenol and chloroform. The use of our specially formulated Lysis Buffer R and Lysing Matrix A in combination with FastPrep instruments from MP Biomedicals enables highly efficient lysis of tissue samples within seconds. DNA, RNA, and proteins are then sequentially purified from the same lysate. Briefly, DNA is absorbed onto the first spin column, while the flow-through is collected and used for RNA purification. The second spin column captures RNA and the flow-through from this step is used for protein extraction. DNA and RNA are bound on the first and second columns, respectively, and then washed and eluted. In the final extraction, proteins are precipitated out of solution, pelleted down by centrifugation, washed, and resuspended. Each molecular component is then immediately available for their respective downstream applications.
Performance Data
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M: DNA marker |
Figure 1: (A) DNA; (B) RNA; (C) protein extracted from each animal tissue using SPINeasy DNA/RNA/Protein All-In-One Kit, analyzed using gel electrophoresis.
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M: DNA marker |
Figure 2: (A) PCR amplification of DNA and (B) RT-PCR amplification of RNA extracted from various samples using SPINeasy DNA/RNA/Protein All-In-One Kit.
Application Notes
The SPINeasy DNA/RNA/Protein All-In-One Kit quickly and high-efficiently isolate DNA, RNA and protein from the same sample, without the use of toxic substances.
Usage Statement
Research Use Only (RUO).
Key Applications
PCR, RT-PCR, cDNA synthesis, NGS, Western blots, ELISA , Genomics, Molecular Biology
| SKU | 116544050 |
| Alternate Names | SPINeasy DNA/RNA/Protein Extraction Kit; Multianalyte Extraction Kit |
| Application Notes | The SPINeasy DNA/RNA/Protein All-In-One Kit quickly and high-efficiently isolate DNA, RNA and protein from the same sample, without the use of toxic substances. |
| Base Catalog Number | 65440 |
| Product Line | SPINeasy |
| Components | 50x Lysing Matrix A, 60 mL Lysis Buffer R, 30 mL Wash Buffer D, 12 mL Wash Buffer R, 10 mL Elution Buffer GD, 10 mL Nuclease-free water, 1 vial DNase I, 5 mL DNase I Buffer, 80 mL Protein Precipitant, 100x Column A with collection tube |
| Format | Kit |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Blood, Cell Culture, Animal Tissues, Swabs |
| Storage | Store at Room Temperature (15-25 °C) in a dry environment |
| Usage Statement | Research Use Only (RUO). |
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