SPINeasy DNA Kit for Water is a high-performance water genomic DNA extraction kit based on silica membrane spin column technology. The kit has been specifically designed to concentrate microbes present in water samples by filtration and transfer to a Lysing Matrix tube (4.5 mL) for the best extraction of microbial gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of microbial cells, including bacteria, fungi, virus, and protozoans. Column W1 (provided with the kit) has high binding capacity and selectivity for gDNA. The combination of components in the kit helps extract microbial gDNA of high yield and purity that is ready for downstream applications, such as restriction digestion, PCR, and NGS.
Performance Data
SPINeasy DNA Kit for Water has consistently achieved better yield along with high purity as compared to competitors’ kits. Total yield is calculated by multiplying the DNA concentration by total purified sample volume, and integrity is determined by absence of visible shearing or smearing of DNA bands during gel electrophoresis.
Results: SPINeasy DNA Kit for Water consistently achieved better yield across various types of water samples as compared to competitors’ DNA kits.
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M: 1kb plus DNA ladder |
Results: SPINeasy DNA Kit for Water resulted in more intact bands of extracted DNA as compared to competitors’ DNA kits.
Application Notes
Extract gDNA of high yield and purity from different types of water including river water, pond water, seawater, sewage that is ready for downstream analyses such as PCR, restriction digestion and sequencing.
Usage Statement
Research Use Only (RUO).
Key Applications
Genomic research; Environmental study
| SKU | 116536050 |
| Alternate Names | SPINeasy DNA Extraction Kit for Water; gDNA Extraction Kit for Water |
| Application Notes | Extract gDNA of high yield and purity from different types of water including river water, pond water, seawater, sewage that is ready for downstream analyses such as PCR, restriction digestion and sequencing. |
| Base Catalog Number | 6536050 |
| Product Line | SPINeasy |
| Components | 50 x 5 mL Lysing Matrix E, 60 mL Lysis Buffer W1, 8 mL Lysis Buffer W2, 550 μL RNase A Solution, 15 mL Inhibitor Removal W, 30 mL Binding Buffer W, 9 mL Wash Buffer W1, 6 mL Wash Buffer W2, 10 mL DES Buffer, 50 x Filter Membrane, 50 x Column W1, 50 x 2.0 mL Collection Tubes, 50 x 1.5 mL Collection Tubes |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Wastewater, Environmental Water, Water samples including fresh and sea water, pond and river water, wastewater, etc. |
| Storage | Store at Room Temperature (15-30 °C), Dry environment. |
| Usage Statement | Research Use Only (RUO). |
![MP COA [116536050] lot ID: FC080B](https://www.mpbio.com/static/version1747228034/frontend/Mpbio/default/en_US/images/document.png "MP COA [116536050] lot ID: FC080B")
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