Product Description
SARS-CoV-2 Spike RBD Mouse Monoclonal Antibody
Application Notes
ELISA, WB, IF
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, WB, IF
Specifications
| SKU | 08L100002 |
| Alternate Names | S1-RBD protein, NCP-CoV RBD Protein, novel coronavirus RBD Protein, 2019-nCoV RBD Protein, S glycoprotein Subunit1 RBD Protein |
| Antibody Type | Monoclonal Antibody |
| Application Notes | ELISA, WB, IF |
| Background_information | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. S1 mainly contains a receptor-binding domain (RBD), which is responsible for recognizing the cell surface receptor. The main functions of the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; the Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor-binding domain (RBD), leading to a higher mortality rate. |
| Bioreactivity | High specificity against SARS-CoV-2 antigen has been experimentally validated. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Formulation Details | In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
| Grade | Molecular Grade |
| Host | Mouse |
| Immunogen | Immunogen is a full-length SARS-CoV-2 Spike RBD protein |
| Isotype | Mouse IgG |
| Molecular Weight | Observed at 30kDa |
| Pack Size | 100 µL |
| Preparation Method | Affinity purification |
| Solubility | Dissolves in Tris or PBS buffer immediately |
| Species Reactivity | SARS-CoV-2 |
| Storage | -20° |
| Typical Working Concentration | ELISA: 1:37500 to 1:150000; WB: 1:500 to 1:2000; IF: 1:50 to 1:200. |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Safety Data Sheets
English (United States)
Brochures
Brochures
English (United States)