RPMI 1640, With L-Glutamine, Without Sodium Bicarbonate

RPMI 1640, With L-Glutamine, Without Sodium Bicarbonate

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SKU 0910601-CF
Price:
$24.55
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Description

Product Description

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

Application Notes

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Key Applications

Mammalian Cell Culture

Specifications
SKU 0910601-CF
Application Notes RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. As a modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
Formulation Powder
Applications Mammalian Cell Culture
pH 7.5 - 8.5 (without Sodium Bicarbonate), 7.7 - 8.7 (with Sodium Bicarbonate)
Physical Appearance Light Pink Powder
Presentation powder
Storage and Handling Store at +4°C.