Recombinant SARS-CoV-2 S1+S2 ECD Protein (His tag, pre-fusion state)
Product Description
Recombinant SARS-CoV-2 S1+S2 ECD(S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of SARS-CoV-2 S1+S2 ECD(S-ECD) (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.It is the wildtype sequence in pre-fusion state without any mutation.
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, SPR, SDS-PAGE, Western Blotting, Immunoprecipitation, Immune Blocking, Binding Assay
Specifications
| SKU | 08L100010 |
| Alternate Names | S glycoprotein; S protein; Spike glycoprotein |
| Bioreactivity | Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 S1+S2 ECD(S-ECD) at 2 μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.15-3.72 ng/mL. |
| Concentration | 1 mg/mL if dissolve in 100 µL of ddH20 or biological buffer |
| Conjugate | Unconjugated |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method. |
| Formulation Details | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.3-7.4. |
| Grade | Molecular Grade |
| Host | HEK293 cells |
| Pack Size | 100 μg |
| Preparation Method | Affinity Chromatography |
| Protein Tag | C-6×His |
| Purity | > 97% by SDS-PAGE |
| Solubility | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Addition of a carrier protein (such as 0.05-0.1% BSA) is recommended for long-term storage. |
| Species Reactivity | Human, Mouse, Rat |
| Storage | -20° |
| Uniprot Number | P0DTC2 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Safety Data Sheets
English (United States)
Brochures
Brochures
English (United States)