Product Description
Recombinant SARS-CoV-2 Nucleocapsid Protein with His tag is produced by Baculovirus-Infected Sf9 Cells expression system. The target protein is expressed with sequence (Ser2-Ala419) of SARS-CoV-2 Nucleocapsid protein (Accession #QHD43423.2) fused with a 6×His tag at the N-terminus.
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, SPR, SDS-PAGE, Western Blotting, Immunoprecipitation, Immune Blocking, Binding Assay
Specifications
| SKU | 08L100012 |
| Alternate Names | Nucleocapsid protein; Nucleoprotein; Protein N |
| Bioreactivity | Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid Protein at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 N Protein Rabbit mAb with a linear range of 0.032-0.79 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 46.90 ng/mL. |
| Concentration | Please check the vial’s label |
| Conjugate | Unconjugated |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method |
| Formulation Details | Supplied as a 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH 8.0. |
| Grade | Molecular Grade |
| Host | Baculoviral-Infected Sf9 cells |
| Pack Size | 100 μg |
| Preparation Method | Affinity Chromatography |
| Protein Tag | N-6×His |
| Purity | > 97% by SDS-PAGE |
| Solubility | Reconstitute it with sterile PBS or 10mM Tris buffer. Addition of a carrier protein (such as 0.05-0.1% BSA) is recommended for long-term storage. |
| Species Reactivity | Human, Mouse, Rat |
| Storage | -20° |
| Uniprot Number | P0DTC9 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Safety Data Sheets
English (United States)
Brochures
Brochures
English (United States)