Product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase. This product is a conjugate of Protein A and highly purified (Rz >3.0) horseradish peroxidase. Protein A is a component of the cell wall of Staphylococcus aureus (Cowan 1 Strain), and binds to the Fc portion of immunoglobulin molecules of most mammalian species. It is composed of a single polypeptide chain of molecular weight 42,000. Protein A binds IgG molecules and, in some cases, IgM and IgA.
Application Notes
Peroxidase-Conjugated Protein A is a versatile reagent which is useful in many immunological assay systems. It is intended for use in cell and tissue staining, dotting and blotting, ELISA and other immunoassays. For each specific experiment, the product should be titered to maximize signal to background.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Dot Blot | ELISA | Immunoblot | Immunohistochemistry
SKU | 0855901 |
Alternate Names | Protein A, HRP conjugate |
Application Notes | Peroxidase-Conjugated Protein A is a versatile reagent which is useful in many immunological assay systems. It is intended for use in cell and tissue staining, dotting and blotting, ELISA and other immunoassays. For each specific experiment, the product should be titered to maximize signal to background. |
Base Catalog Number | 55901 |
Conjugate | HRP |
Formulation Details | Purified |
Pack Size | 2 mg |
Preparation Method | The product is in 10mM phosphate, 0.15M NaCl buffer, pH 7.4, 1% ovalbumin, 40% glycerol containing 0.1% proclin as preservative. |
Purity | Immunoaffinity Chromatography |
Species Reactivity | Rabbit, Sheep, Mouse, Pig, Rat, Dog, Goat, Cow, Binding Characteristics of Protein A: Human, Guinea Pig |
Typical Working Concentration | Suggested Trial Dilutions: 1:100 - 1:200; 1:8,000 - 1:16,000 |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |