MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-32™)
SKU: 117040100
The Ready-to-Use MagBeads Plasmid Miniprep Kit offers a scalable and automatable purification method using magnetic bead technology, suitable for extracting both high and low copy number plasmids. This kit efficiently yields plasmid DNA (2 - 20 µg) from 0.5 - 5 mL of overnight E. coli cultures, utilizing paramagnetic beads for rapid processing. Its magnetic technology and Ready-to-Use format enhance user-friendliness, making it ideal for high-throughput automated nucleic acid purification.
The kit includes components for cell lysis and neutralization. Bacterial cells are first resuspended in Resuspension Buffer. Following resuspension, the cells are lysed with Alkaline Lysis Buffer to release plasmid DNA into the solution. Contaminants such as proteins and chromosomal DNA are precipitated by the Neutralization Buffer. The supernatant, which contains the plasmid DNA, binds to the magnetic particles, and a subsequent wash with an alcohol-containing buffer effectively removes impurities, resulting in purified plasmid DNA.
This kit is compatible with the MPure-32™ aNAP System, or other compatible high-throughput instruments, facilitating a faster extraction process that takes approximately 40 - 50 minutes.
Performance
Figure 1. Agarose gel electrophoresis of (a) pWPI and (b) pBBRMCS-2 plasmid extracted using MagBeads Plasmid Miniprep Kit and Competitor O. Lane M: DNA marker. Lane 1 - 2: Manual extraction using MagBeads Plasmid Miniprep kit. Lane 3 - 4: Manual extraction using Competitor O kit.
Figure 2. Downstream applications of plasmids extracted using the MagBeads Plasmid Miniprep Kit (as shown by agarose gel electrophoresis):
(a) PCR amplification
Lane 1–2: PCR products using manually extracted plasmid DNA as template.
Lane 3: Negative control (no template).
Lane M: DNA marker.
(b) Restriction digestion with EcoRI and NotI
Lane 1: Uncut plasmid.
Lane 2: Plasmid digested with EcoRI and NotI.
Lane M: DNA marker.
Application Notes
MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-32™)采用磁珠法核酸提取技术，适用于高拷贝数和低拷贝数质粒的高通量和自动化提取
Usage Statement
Research Use Only (RUO)
Key Applications
PCR, restriction digestion
| SKU | 117040100 |
| Application Notes | MagBeads Plasmid Miniprep Kit (Ready-to-Use for MPure-32™)采用磁珠法核酸提取技术，适用于高拷贝数和低拷贝数质粒的高通量和自动化提取 |
| Base Catalog Number | 7040100 |
| Product Line | MagBeads |
| Compatible Instruments | MPure-32™ |
| Components | Resuspension Buffer MN1 (26 mL), Alkaline Lysis Buffer MN2 (26 mL), Neutralization Buffer MN3 (37 mL), Reagent Plate MN (6 × 96 Preps), Elution Buffer MN (8 mL), RNase A (260 µL), Indicator Solution (130 µL), 8 Strips Tip Comb (12 Pieces) |
| Format | Kit |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store Resuspension Buffer MN1 at 2-8˚C after adding RNase A, the remaining kit contents should be stored at 15-25°C. |
| Usage Statement | Research Use Only (RUO) |
Powered by Bioz