MagBeads FastRNA Kit for Feces (Ready-to-Use for MPure-32)
SKU: 117040300
MagBeads FastRNA Kit for Feces (Ready-To-Use for MPure-32) is a scalable and automatable magnetic bead-based RNA purification technology designed for total isolation of RNA from feces samples. This kit is recommended to be used with MPure-32 (Cat. No. EMC043). The composition of feces samples is largely dependent on diet which includes fibers, undigested particles, bilirubin, complex polysaccharides, and lipids. These compounds impair sample homogenization, decreasing both the quantity and quality of the extracted RNA.
The MagBeads FastRNA Kit for Feces integrates our 1) state-of-the-art beads-beating technology, 2) a lysis chemistry compatible with the widest range of samples and 3) our proprietary inhibitor removal expertise. A new technology of selective binding of RNA along with our FastDNase I Enzyme treatment allows the complete removal of DNA. The MagBeads FastRNA Kit for Feces simplifies the RNA purification process by eliminating complications associated with diverse fecal sample types, making it hassle-free and time efficient. Contaminated DNA is removed by a DNase I treatment step resulting in high yield and purity. The extracted RNA is compatible with downstream applications such as RT-qPCR amplification without the need of further inhibitor/DNA removal steps.
Performance
Figure 1. Gel Electrophoresis and Quality Assessment of RNA Extracted from Different Species Using the MagBeads FastRNA Kit for Feces. This figure shows the gel electrophoresis and quantitative assessment of RNA extracted from different fecal sources using the MagBeads FastRNA Kit for Feces. The samples represent omnivore feces (lanes 1–4), human feces (lanes 5–8), and herbivore feces (lanes 9–12).
Figure 2. Performance evaluation of RNA extracted from human fecal samples using the MagBeads FastRNA Kit for Feces (MP) compared to Competitor M. RNA was independently extracted from human fecal samples (two extractions per sample, each dot on the plot represents one extraction). The RNA yield, purity (A260/A230 ratio) and integrity were assessed using spectrophotometry and agarose gel electrophoresis.
Figure 3: Performance evaluation of RNA extracted from animal fecal samples using the MagBeads FastRNA Kit for Feces (MP) compared to Competitor M. RNA was independently extracted from animal feces samples (two extractions per sample, each dot on the plot represents one extraction). The RNA yield, purity (A260/A230 ratio) and integrity were assessed using spectrophotometry and agarose gel electrophoresis. In addition, the extracted RNA was also assessed for its amplifiability using qPCR and the Ct values given by MP are earlier than Competitor M. The horizontal bars indicate the median value.
Application Notes
The MagBeads FastRNA Kit for Feces (Ready-To-Use for MPure-32) was designed for rapidly isolating RNA from Feces using Magnetic Beads automation technology.
Usage Statement
Research Use Only (RUO)
Key Applications
RT-PCR
| SKU | 117040300 |
| Application Notes | The MagBeads FastRNA Kit for Feces (Ready-To-Use for MPure-32) was designed for rapidly isolating RNA from Feces using Magnetic Beads automation technology. |
| Base Catalog Number | 7040300 |
| Components | Lysing Matrix YB (96 x 2 mL), DNase I (1.2 mL), DNase I Buffer (27 mL), Magnetic Beads F (3 mL), Buffer PC (22 mL), Buffer MFR1 (55 mL), Buffer MFR2 (5.5 mL), Reagent Plate MFR (16 PREPS*6), 8 Strips Tip Comb (12 pieces). |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store DNase I, Buffer MFR2, Magnetic Beads F, and Buffer PC at 2-8˚, the remaining kit contents should be stored at 15-25°C. |
| Usage Statement | Research Use Only (RUO) |
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