The MagBeads FastRNA™ Kit is designed to quickly and effectively isolate high-quality total RNA from tissues and cultured cells in 60 minutes. This kit allows for a higher sample throughput (up to 96 samples) when used with FastPrep-96 (see Cat. No. 116010500) and Lysing Matrix A or D, 96-tube racks (see Cat. No. 116980001 and 116983001) to efficiently lyse all cells and tissues. The cleared lysate can then be transferred to tubes or a 96-deep well plate. After addition of magnetic beads and binding solution, RNA is adsorbed onto the surface of the beads, and impurities such as proteins and other inhibitors are washed away.
This kit is versatile and can be used with most automated nucleic acid extraction platforms available in the market. Alternatively, extractions can be performed manually using microcentrifuge tubes (see Cat. No. 115081500 and 115083500) or 96-deep well plates (see Cat. No. 115095050) with a magnetic stand (see Cat. No. 116570413, 116570426, and 116570429).
The kit is equipped with reagents specially formulated to remove common inhibitory substances found in biological samples, such as proteins and enzyme inhibitors. As a result, the output is highly pure total RNA that is suitable for a variety of molecular biology applications, including RT-PCR, viral RNA testing by PCR/qPCR, transcriptomics, NGS, and other RNA-based assays.
Performance Data
Figure 1. Measurements of nucleic acid purity for total RNA extracted from 5 mg and 20 mg of chicken liver samples using the MagBeads FastRNA Kit. Purity of extracted total RNA was determined using absorbance ratios, A260/280 and A260/230. A260/280 values (orange bars) of 1.8 – 2.1 is considered an indicator of pure RNA without protein contamination. A260/230 ratios (blue bars) ranging from 1.85 to 2.05 are generally considered optimal, indicating removal of unwanted organic compounds and other cellular impurities.
Figure 2. Gel electrophoresis of total RNA extracted from 5 mg and 20 mg of chicken liver samples. Intact and bright ribosomal RNA (rRNA) bands indicate high integrity of extracted RNA.
Application Notes
Magbeads Fast RNA Kit utilizes magnetic bead-based technology for convenient and fast purification of total RNA from cultured cells and a variety of animal, plant, fungal, and other cell/tissue samples.
Usage Statement
Research Use Only (RUO)
Key Applications
Total RNA extracted from MagBeads FastRNA Kit can be used in several downstream applications, including RT-PCR, Viral RNA testing, Transcriptomics, and more
| SKU | 116572096 |
| Alternate Names | Magnetic Bead RNA Extraction Kit; High-throughput RNA Extraction; 96-well RNA extraction Kit |
| Application Notes | Magbeads Fast RNA Kit utilizes magnetic bead-based technology for convenient and fast purification of total RNA from cultured cells and a variety of animal, plant, fungal, and other cell/tissue samples. |
| Base Catalog Number | 6572096 |
| Product Line | MagBeads |
| Components | 4 mL Magnetic beads, 48 mg Proteinase K, 5 mL Protease Dissolve Buffer, 2 x 600 µL DNase I, 2 x 30 mL DNase Buffer, 80 mL Buffer RTL, 30 mL Buffer MCB, 66 mL Buffer MW1, 50 mL Buffer RW2, 30 mL RNase-free Water |
| Format | Kit |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Face shield; Gloves |
| Sample Type | Cell Culture, Animal Tissues, Fungi, Plant Tissue |
| Sample Volume | Animal Tissue (20 mg), Plant Tissue (50 mg), Fungi (5 X 10^6), Cultured Cells (1 X 10^7) |
| Storage | Store at Room Temperature (15-30 °C) in a dry environment. Store Magnetic Beads at 2-8 ℃ upon arrival; do not freeze. |
| Usage Statement | Research Use Only (RUO) |
Powered by Bioz