MagBeads FastDNA® Kit for Blood, 96 preps
SKU: 116574096
MagBeads FastDNA® Kit for Blood is designed to quickly and effectively isolate high-quality genomic DNA (gDNA) from whole blood, lymphocytes, cultured cells, plasma, serum, buffy coat, bone marrow, and other bodily fluids. Samples are lysed and digested with proteases, thus releasing the DNA into the lysate. After addition of magnetic beads and binding solution, DNA is adsorbed onto the surface of the beads, and impurities such as proteins, salts, and other inhibitors are washed away. As a result, extracted gDNA is highly pure and suitable for PCR/qPCR, NGS, and other molecular biology applications.
This kit is versatile and can be used with most automated nucleic acid extraction platforms available in the market. Alternatively, extractions can be performed manually using microcentrifuge tubes (see Cat. No. 115081500 and 115083500) or 96-deep well plates (see Cat. No. 115095050) with a magnetic stand (see Cat. No. 116570413, 116570426, and 116570429).
Performance Data
The MagBeads FastDNA Kit for Blood has been developed to accommodate a wide range of blood samples. The purity of extracted DNA was determined from various blood samples at different temperatures by measuring absorbance ratios (A260/230 and A260/280).
Figure 1. A total of 8 fresh and frozen blood samples were subjected to extraction using the MagBeads FastDNA Kit for Blood. Each extraction used 200 µL of starting material. The results show that temperature does not have a significant effect on purity.
Application Notes
MagBeads FastDNA Kit for Blood is intended for extraction and purification of genomic DNA from whole blood and other bodily fluids. Isolated DNA is ready for sensitive molecular biology applications, including PCR, qPCR, NGS, Southern Blotting, Arrays, DNA Methylation, and more.
Usage Statement
Research Use Only (RUO)
Key Applications
Genomics, Molecular Biology, PCR, qPCR, NGS, Shotgun sequencing
| SKU | 116574096 |
| Alternate Names | Magnetic Bead DNA Extraction Kit for Blood, High-throughput DNA Extraction Kit for Blood, 96-well DNA Extraction Kit for Blood |
| Application Notes | MagBeads FastDNA Kit for Blood is intended for extraction and purification of genomic DNA from whole blood and other bodily fluids. Isolated DNA is ready for sensitive molecular biology applications, including PCR, qPCR, NGS, Southern Blotting, Arrays, DNA Methylation, and more. |
| Base Catalog Number | 6574096 |
| Product Line | MagBeads |
| Components | 3.5 mL Magnetic Beads, 50 mg Proteinase K, 3 mL Protease Dissolve Buffer, 60 mL Buffer MLA, 120 mL Buffer DW1, 60 mL Buffer CW, 60 mL Buffer BW3, 30 mL Elution Buffer |
| Format | Kit |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Face shield; Gloves |
| Sample Type | Plasma, Serum, Blood, Bodily Fluid |
| Storage | Store at Room Temperature (15-30 °C) in a dry environment. Store Magnetic Beads at 2-8 ℃ upon arrival; do not freeze. |
| Usage Statement | Research Use Only (RUO) |
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