Mouse anti-GAPDH mAb (High Potency)

$243.10
In stock

SKU:  08L100030

Key features and details

This GAPDH Mouse mAb (High Potency) offers high specificity and potency and consistency against GAPDH expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) assays.
Synonyms
G3PD; GAPD; HEL-S-162eP; GAPDH
Molecular Weight:
Observed at 36KDa
Packaging size:
100 µL
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Product Description

MP Biomedicals GAPDH Mouse mAb (High Potency) offers high specificity and potency and consistency against GAPDH expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) assays.

Usage Statement

Research use only, not for direct human use.

Key Applications

Western blotting (WB), Immunohistochemistry (IHC) and Immunofluorescence (IF) assays

Specifications
SKU 08L100030
Alternate Names G3PD; GAPD; HEL-S-162eP; GAPDH
Antibody Type Monoclonal Antibody
Background_information This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophages. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Base Catalog Number L100030
Bioreactivity This GAPDH Mouse mAb (High Potency) offers high specificity and potency and consistency against GAPDH expressed by human, mouse, and rat species, which has been experimentally validated in Western blotting (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF) assays.
Concentration Please check the vial’s label
Conjugate Unconjugated
Formulation Details In PBS buffer with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3-7.4. This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. This antibody is stable for twelve months from the date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Grade Molecular Grade
Host Mouse
Immunogen Immunogen is a full-length recombinant protein of human GAPDH
Isotype IgG
Molecular Weight Observed at 36KDa
Pack Size 100 µL
Preparation Method Affinity purification
Solubility Dissolves in Tris or PBS buffer immediately
Species Reactivity Human, Mouse, Rat
Specificity High specificity against antigens
Storage -20°
Typical Working Concentration WB: 1:50000 - 1:200000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
Uniprot Number P04406
Usage Statement Research use only, not for direct human use.
Documents

Certificate of Analysis (COA)

Datasheet

Brochures

Brochures
English (United States)