Culture Media

276 Results Found

TRANSFORM & GROW BACTERIAL TRA

- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time

EZ-YEAST TRANSFORMATION KIT

- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA

CIRCLEGROW (STERILE SOLUTION)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

ALKALI-CATION YEAST TRANSFORMA

The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.

Alpha Modification Of Eagle's Medium (AMEM), Powder, With L-Glutamine, Without Deoxyribosides, Ribosides, Sodium Bicarbonate

Alpha Modification of Eagle's Medium (AMEM), powder, with L-glutamine, without deoxyribosides, ribosides, sodium bicarbonate. Originally used for growth of hamster kidney cells.

Ham's F-12 (Modified) With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells. MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.

Hanks' Balanced Salts, Powder, Without Sodium Bicarbonate

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Phosphate Buffered Saline (PBS), Dulbecco's Formula, Powder, Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Hoagland's Modified Basal Salt Mixture

Hoagland's Modified Basal Salt Mixture includes the macro- and micronutrients as described by Hoagland and Arnon, 1950.

Newborn Bovine Serum (NBS) 

This serum is produced from blood collected from newly born calves between 1-14 days of age.

Rabbit Serum

This serum is derived from the blood of healthy adult animals. Rabbit serum is used to support cultures of Borrelia burgdorferi, the causative agent of Lyme disease.

Murashige and Skoog Medium, Without Sucrose, Agar, Iaa, and Kinetin

Murashige and Skoog Medium contains Murashige and Skoog salts, as well as a mixture of vitamins. This modification of the original formula does not contain agar, sucrose, IAA, and Kinetin. Murashige and Skoog Medium is widely used plant tissue culture growth medium. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.

Phosphate Buffered Saline (PBS) (1X Solution) Dulbecco's Formula Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Murashige and Skoog Basal Salt Mixture

Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.

Murashige & Skoog Basal Medium

Murashige and Skoog basal salt medium includes the macro- and micronutrients and vitamins as described by Murashige and Skoog, 1962. It supports or facilitates plant growth and/or shoots proliferation in two or more plant tissue cultures. Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.

Murashige & Skoog Plant Salt Mixture Without Agar

Murashige and Skoog Medium contains Murashige and Skoog salts, as well as a mixture of vitamins. This modification of the original formula does not contain agar. Murashige and Skoog Medium is widely used plant tissue culture growth medium. It has been proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.

CELLect Fetal Bovine Serum, Silver, Mexico Origin

CELLect™ Silver is collected from a U.S.D.A. regulated and monitored abbatoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration with multiple 0.1 micron filters. The care and diligence taken in handling the fetal serum minimizes cell lysis and other detrimental factors thereby reducing possible lysozyme and hemoglobin contamination. This translates into increased performance in the final product. It is supplied sterile, with no detectable mycoplasma or viral infection.