TRANSFORM & GROW BACTERIAL TRA
- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time
EZ-YEAST TRANSFORMATION KIT
- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA
CIRCLEGROW (STERILE SOLUTION)
Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.
ALKALI-CATION YEAST TRANSFORMA
The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.
Cryopres™ Dimethyl Sulfoxide (>99.9% USP DMSO for cryopreservation)
Cryopres™ Dimethyl Sulfoxide is commonly used in cell cryopreservation to prevent damage to the cell membrane during freezing.
Casein Hydrolysate Enzymatic
Casein hydrolysate enzymatic is a pancreatic hydrolysate of casein. This hydrolysate contains, in the form of mixed amino acids and peptides, all amino acids originally present in casein. It is a refined product providing high quality soluble source of amino acids,polypeptides and peptides.