3,3-Diaminobenzidine Tetrahydrochloride

BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is ready to use solution.

(5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting procedures only. Concentration: 0.577 mmol/liter BCIP; 0.122 mmol/liter NBT.

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Stabilized Chromogen Solution
This alkaline phosphatase chromogen solution contains the two components in a stabilized solution which may be used as the final step in color development. The resulting product is a permanent, deep-purple stain. It enhances results in both ELISA and immunohistology assays utilizing peroxidase and alkaline phosphatase.

Used in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. Insoluble end product is resistant to alcohol and fading, making it ideal for immunohistochemistry.