G418 Sulfate Solution

G418 Sulfate Solution

Synonyms Antibiotic G418; Geneticin®

CAS Number: 108321-42-2 Molecular Formula: C20H40O10N4·2H2SO4 Molecular Weight: 692.7

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SKU 0916725-CF
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$149.05
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Description

Product Description

G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.

Application Notes

G418 Sulfate is used as a selective antibiotic in the concentration range of 100 - 200 µg/mL for bacteria, or 200 – 500 µg/mL for most mammalian cells. It is also used for the selection of transfected mammalian cells, yeast, dictyostelium, plant and bacteria.

Key Applications

Cell culture applications | Antimicrobial Treatment | Eukaryotic Selection | Stable Cell Line Generation

Specifications
SKU 0916725-CF
Application Notes G418 Sulfate is used as a selective antibiotic in the concentration range of 100 - 200 µg/mL for bacteria, or 200 – 500 µg/mL for most mammalian cells. It is also used for the selection of transfected mammalian cells, yeast, dictyostelium, plant and bacteria.
Concentration Concentration (active G418 sulfate) 50 ± 1 mg/mL
Formulation Details Liquid
Applications Cell culture applications | Antimicrobial Treatment | Eukaryotic Selection | Stable Cell Line Generation
Molecular Formula C<sub>20</sub>H<sub>40</sub>O<sub>10</sub>N<sub>4</sub>·2H<sub>2</sub>SO<sub>4</sub>
Molecular Weight 692.7
pH 5.3 (Lit.)
Physical Appearance Clear; Colorless Solution
Presentation Solution
Solubility Soluble in water
Sterility Sterile | Filtered through .22 µm micron filter
Storage and Handling Store at +4°C.
Typical Working Concentration 100 - 200 µg ⁄ mL bacteria, 200 – 500 µg ⁄ mL mammalian cells