Biotinylated Recombinant SARS-CoV-2 Spike RBD Protein (C-His-Avi tag)
Product Description
Biotinylated Recombinant SARS-CoV-2 Spike RBD Protein is produced by Expi293 expression system. The target protein is expressed with sequence (Arg319-Asn532) of SARS-CoV-2 Spike RBD fused with His tag and Avi at the C-terminal.
Usage Statement
Research use only, not for direct human use.
Key Applications
ELISA, SPR, SDS-PAGE, Western Blotting, Immunoprecipitation, Immune Blocking, Binding Assay
Specifications
| SKU | 08L100009 |
| Alternate Names | S protein RBD; Spike glycoprotein Receptor-binding domain; S glycoprotein RBD; Spike protein RBD |
| Bioreactivity | Immobilized Human ACE2 at 2 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated SARS CoV-2 Spike RBD with the EC50 of 0.097 μg/mL determined by ELISA. |
| Concentration | 1 mg/mL if dissolve in 100 µL of ddH20 or biological buffer |
| Conjugate | Biotinylated |
| Endotoxin | < 0.1 EU/μg of the protein by LAL method |
| Formulation Details | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.3-7.4. |
| Grade | Molecular Grade |
| Host | Expi293 cells |
| Pack Size | 100 μg |
| Preparation Method | Affinity Chromatography |
| Protein Tag | C-His-Avi |
| Purity | >97% by Tris-Bis PAGE ;> 95% by SEC-HPLC |
| Solubility | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Addition of a carrier protein (such as 0.05-0.1% BSA) is recommended for long-term storage. |
| Species Reactivity | Human, Mouse, Rat |
| Storage | -20° |
| Uniprot Number | QHD43416.1 |
| Usage Statement | Research use only, not for direct human use. |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
English (United States)
Safety Data Sheets
English (United States)
Brochures
Brochures
English (United States)