FastDNA-96 Plant/Seed DNA Kit

FastDNA-96 Plant/Seed DNA Kit

Synonyms 96 well DNA purification kit

SKU 119696600
Price:
$1,195.00
In Stock
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Description

Product Description

Rapid, High-Throughput Isolation of PCR-Ready Genomic DNA from Plant and Seed Samples The FastDNA™-96 Plant and Seed DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from vegatative samples in approximately 50 minutes. Plant samples including stems, roots, leaves, buds, flowers, fruits and seeds are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Up to 80 mg samples are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack ( 96-deep well plate) containing 2.0 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse a wide variety of plant specimens, while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes tannins, polyphenols and polysaccharides that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, arrays and PCR (A260/A280 ratios ≥ 1.8). Yield is typically 5 μg of total DNA eluted in 50-100 μL of elution solution. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.

Application Notes

The FastDNA™-96 Plant/Seed DNA Kit for FastPrep® -96 is used for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, and seeds. Eluted DNA can be used for downstream molecular-based applications including PCR, arrays, digestion, electrophoresis etc.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

High-Throughput; Rapid Isolation of PCR-Ready Genomic DNA from Plant and Seed Samples using the FastPrep-96™ System

Specifications
SKU 119696600
FastPrep Instruments Models 2 x 96 Well Adapter
Alternate Names 96 well DNA purification kit
Application Notes The FastDNA™-96 Plant/Seed DNA Kit for FastPrep® -96 is used for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, and seeds. Eluted DNA can be used for downstream molecular-based applications including PCR, arrays, digestion, electrophoresis etc.
Brand FastDNA™
Certifications DNase and RNase Free Lysing Matrix Racks
Components FastDNA-96&trade||Lysing Matrix Rack (2.0 mm Beads) 2 x 96-well rack||Lysis Buffer 2 x 40 mL||Plant/Seed DNA Binding Solution 150 mL||Binding Plate Pre-Wash Buffer 50 mL||Plant/Seed DNA Wash Buffer 100 mL||Elution Solution 2 x 10 mL||Inhibitor Plate Prep Solution
Hardness Mohs 9, Rockwell A >87, Vickers >1350
Applications High-Throughput; Rapid Isolation of PCR-Ready Genomic DNA from Plant and Seed Samples using the FastPrep-96™ System
Material of Construction Matrix Z, 2.0 mm diameter Yttria-Stabilized Zirconium Oxide
Materials Required Not Supplied FastPrep-96™ Instrument or alternative high speed disruptor, Centrifuge with a swing-bucket style rotor that can spin up to 3,500 – 5,000 rpm, Swing-bucket centrifuge microplate adaptors for 96-well plates (pair), Plate shaker, Sterile water
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Product Overview The FastDNA™-96 Plant and Seed DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from plant samples in approximately 50 minutes. Plant samples including stems, roots, leaves, buds, flowers, fruits and seeds are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Procedure of DNA isolation can be performed in minutes, and there is no need for organic denaturants or proteinases.
Research Areas OTHER - Drug Discovery|| OTHER - Soil & Environmental Science
Sample Type Plants||Seeds||Stems||Roots||Leaves||Buds||Flowers||Fruits
Sample Volume up to 80 mg
Specific Gravity 6.02
Storage and Handling Store at Room Temperature(15-30 °C).
Tube Compatibility 1.2 mL Cluster Strip, 96 Well Rack
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.