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FastDNA-96 Fecal DNA Kit

FastDNA-96 Fecal DNA Kit

Packaging size: 2 x 96 Preps

SKU 119696400
Price:
$1,195.00
In Stock
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Description

Product Description

The FastDNA-96™ Fecal DNA Kit isolates bacterial, protist, or host DNA from up to 80 mg of wet or dry stool, and even from caecum samples, through a purification process that eliminates PCR inhibitors. Fecal microbes, fungi, parasites, and other stool organisms are first lysed in approximately 60 seconds with the FastPrep-96™ Instrument.

Application Notes

The FastDNA-96™ Fecal DNA Kit quickly and efficiently isolates inhibitor-free, PCR-ready genomic DNA from stool samples (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used for Isolation of PCR-Ready Genomic DNA from Fecal Samples

Specifications
SKU 119696400
FastPrep Instruments Models 2 x 96 Well Adapter
Application Notes The FastDNA-96™ Fecal DNA Kit quickly and efficiently isolates inhibitor-free, PCR-ready genomic DNA from stool samples (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.).
Components FastDNA-96™ Lysing Matrix Rack (0.5 mm Beads) 2 x 96-well rack, Lysis Buffer 2 x 40 mL, Fecal DNA Binding Solution 150 mL, Binding Plate Pre-Wash Buffer 50 mL, Fecal DNA Wash Buffer 100 mL, Elution Solution 2 x 10 mL, Inhibitor Plate Prep Solution 30 mL, Deep-Well Plate 2 each, MP-96 Binding Plate 2 each, MP-96 Inhibitor Removal Plate 2 each, Collection Plate 2 each, Elution Plate 6 each, Foil Plate Cover 4 each.
Applications Used for Isolation of PCR-Ready Genomic DNA from Fecal Samples
Material of Construction Matrix Y, 0.5 mm diameter Yttria-Stabilized Zirconium Oxide
Materials Required Not Supplied FastPrep-96™ Instrument or alternative high speed disruptor, Centrifuge with a swing-bucket style rotor that can spin up to 3,500 – 5,000 rpm, Swing-bucket centrifuge microplate adaptors for 96-well plates (pair), Plate shaker, Sterile water.
Molecule Enriched DNA
Pack Size 2 x 96 Preps
pH Reagent: Lysis Buffer Lysis Buffer-pH: 8.0, Reagent: Fecal DNA Binding Solution Fecal DNA Binding Solution- pH Range: 8.5 - 9.5, Reagent: Binding Plate Pre-Wash Buffer Binding Plate Pre-Wash Buffer- pH Range: 7.0 - 8.0, Reagent: Fecal DNA Wash Buffer Fecal DNA Wash Buffer- pH Range: 7.5 - 8.0, Reagent: Elution Solution Elution Solution- pH Range: 7.0 - 8.0, Reagent: Inhibitor Plate Prep Solution Inhibitor Plate Prep Solution- pH Range: 6.0 - 7.0.
Product Overview Up to 80 mg of wet or dry feces are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack (96-deep well plate) containing 0.5 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse a wide variety of eukaryotic and prokaryotic organisms present in mammalian stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.), while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes humic acids, polyphenols that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, methylation and PCR (A260/A280 ratios = 1.8). Yield is typically 5 µg of total DNA eluted in 50-100 µL of elution solution. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
Sample Type mammalian stool (i.e. humans; primates; birds; rodents; cattle; canine; feline; porcine; etc.)
Storage and Handling All FastDNA-96® Fecal DNA Kits are shipped ambiently. All FastDNA-96® Fecal DNA Kit reagents are stable at room temperature (15-30 °C).
Tube Compatibility 1.2 mL in 96 Deep Rack
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.