FastDNA-96 Fecal DNA Kit
The FastDNA-96™ Fecal DNA Kit isolates bacterial, protist, or host DNA from up to 80 mg of wet or dry stool, and even from caecum samples, through a purification process that eliminates PCR inhibitors. Fecal microbes, fungi, parasites, and other stool organisms are first lysed in approximately 60 seconds with the FastPrep-96™ Instrument.
The FastDNA-96™ Fecal DNA Kit quickly and efficiently isolates inhibitor-free, PCR-ready genomic DNA from stool samples (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.).
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Used for Isolation of PCR-Ready Genomic DNA from Fecal Samples
|FastPrep Instruments Models||2 x 96 Well Adapter|
|Application Notes||The FastDNA-96™ Fecal DNA Kit quickly and efficiently isolates inhibitor-free, PCR-ready genomic DNA from stool samples (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.).|
|Components||FastDNA-96™ Lysing Matrix Rack (0.5 mm Beads) 2 x 96-well rack, Lysis Buffer 2 x 40 mL, Fecal DNA Binding Solution 150 mL, Binding Plate Pre-Wash Buffer 50 mL, Fecal DNA Wash Buffer 100 mL, Elution Solution 2 x 10 mL, Inhibitor Plate Prep Solution 30 mL, Deep-Well Plate 2 each, MP-96 Binding Plate 2 each, MP-96 Inhibitor Removal Plate 2 each, Collection Plate 2 each, Elution Plate 6 each, Foil Plate Cover 4 each.|
|Applications||Used for Isolation of PCR-Ready Genomic DNA from Fecal Samples|
|Material of Construction||Matrix Y, 0.5 mm diameter Yttria-Stabilized Zirconium Oxide|
|Materials Required Not Supplied||FastPrep-96™ Instrument or alternative high speed disruptor, Centrifuge with a swing-bucket style rotor that can spin up to 3,500 – 5,000 rpm, Swing-bucket centrifuge microplate adaptors for 96-well plates (pair), Plate shaker, Sterile water.|
|pH||Reagent: Lysis Buffer Lysis Buffer-pH: 8.0, Reagent: Fecal DNA Binding Solution Fecal DNA Binding Solution- pH Range: 8.5 - 9.5, Reagent: Binding Plate Pre-Wash Buffer Binding Plate Pre-Wash Buffer- pH Range: 7.0 - 8.0, Reagent: Fecal DNA Wash Buffer Fecal DNA Wash Buffer- pH Range: 7.5 - 8.0, Reagent: Elution Solution Elution Solution- pH Range: 7.0 - 8.0, Reagent: Inhibitor Plate Prep Solution Inhibitor Plate Prep Solution- pH Range: 6.0 - 7.0.|
|Product Overview||Up to 80 mg of wet or dry feces are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack (96-deep well plate) containing 0.5 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse a wide variety of eukaryotic and prokaryotic organisms present in mammalian stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.), while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes humic acids, polyphenols that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, methylation and PCR (A260/A280 ratios = 1.8). Yield is typically 5 µg of total DNA eluted in 50-100 µL of elution solution. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.|
|Sample Type||mammalian stool (i.e. humans; primates; birds; rodents; cattle; canine; feline; porcine; etc.)|
|Storage and Handling||All FastDNA-96® Fecal DNA Kits are shipped ambiently. All FastDNA-96® Fecal DNA Kit reagents are stable at room temperature (15-30 °C).|
|Tube Compatibility||1.2 mL in 96 Deep Rack|