MPure Blood DNA Extraction Kit
MPure Blood DNA Extraction Kit 200 is used with the MPure-12 aNAP System for extraction of DNA from 100-400 µL mammalian whole blood, suspension of mammalian blood cells.
Nucleic acids extracted can be used in a number of downstream applications including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
PCR; qPCR; Sequencing (NGS); Microarray; RFLP; Southern Blot Analysis.
|Alternate Names||MPure Blood DNA Extraction Kit 200|
|Application Notes||Nucleic acids extracted can be used in a number of downstream applications including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.|
|Capacity||100-400 µL whole blood (WBC count less than 2x 10000 cells/µL) ; 100-400µL leukocyte concentration (contain no more than 5 x 106 cells) ; 100-400 µL buffy coat|
|Components||Proteinase K solution: 40 µL |Lysis Buffer 2: 1000 µL |Binding Buffer 1: 600 µL |Magnetic Bead Solution: 800 µL |Washing Buffer 1: 1000 µL |Washing Buffer 2: 1000 µL |Washing Buffer 3: 1000 µL |Elution Buffer 1: 1000 µL |lution Buffer 2: 1000 µL|
|Instrument or System Type||Nucleic Acid Purification|
|Applications||PCR; qPCR; Sequencing (NGS); Microarray; RFLP; Southern Blot Analysis.|
|Kit or Assay Type||Reagent Kit for Nucleic Acid Purification|
|Material of Construction||Plastic |Aluminum Foil |Buffers|
|Materials Required Not Supplied||Pipettes |Pipette Tips |Test Tubes |Gloves|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||Reagent Cartridge |Reaction Chamber |Tip Holder |Filtered Tip |Piercing Pin |Sample Tube (2 mL) |Elution Tube (1.5 mL)|
|Product Overview||MPure Blood DNA Extraction Kit 200 is used with the MPure-12 aNAP System for extraction of DNA from 100-400 µL mammalian whole blood, suspension of mammalian blood cells. Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation |Soil Science |Proteomics |Metagenomics |Metabolomics |Food Sciences |Forensics |Plant Pathology |Communicable Disease |Cancer |Agricultural |Microbiomics |Epigenetics|
|Sample Throughput||1-12 samples and 48 Preps/Kit|
|Sample Type||100-400 µL whole blood (WBC count less than 2x 104 cells/µL) |100-400 µL leukocyte concentration (contain no more than 5x 106 cells) |100-400µL buffy coat|
|Sample Volume||100-400 µL whole blood (WBC count less than 2x 104 cells/µL); 100-400µL leukocyte concentration (contain no more than 5x 106 cells); 100-400µL buffy coat;|
|Storage and Handling||Store at room temperature (15-25°C). Do not freeze the reagent cartridges. The Kits are stable for 12 months under the condition. Store the purified total nucleic acid at 4°C (short-term) or aliquot and store at –70°C (long-term) before perform the downstream analysis.|