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Lysing Matrix A

Lysing Matrix A

Synonyms A Matrix

Molecular Formula: Banded Satellite: ZrO2 88%, CeO 10%, Garnet: Fe3Al2(SiO4)3

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SKU 116910050-CF
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Description

Product Description

Lysing Matrix A 2 mL tube contains garnet matrix and one 1/4" ceramic sphere. Extra 1/4" ceramic spheres are packaged separately. Lysing Matrix A tubes have orange caps.

Application Notes

Lysing Matrix A is used for all sample types, except soil, for the subsequent isolation of genomic DNA and proteins.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Nucleic Acid Isolation, Nucleic Acid Extraction, Proteomics, Purification and Detection, Protein Purification, DNA Purification, Plant Tissue Processing, Tissue Analysis, Bacterial Lysis, Yeast Lysis, Fungal Tissue Analysis, Animal Cell Lysis, Animal Tissue Analysis, Microbiome Studies, Metabolite Extraction, Grind Seeds, Homogenization

Specifications
SKU 116910050-CF
FastPrep Instruments Models QuickPrep (24x2 mL) |HiPrep (48x2 mL) |CoolPrep (24x2 mL) |QuickFlex (96x2 mL) |ConeFlex Legacy (allows all FP24 adapters on FP96) |QuickPrep All Metal (24x2 mL)
Application Notes Lysing Matrix A is used for all sample types, except soil, for the subsequent isolation of genomic DNA and proteins.
Average Size Zirconium sphere, 6.35 mm, Garnet Flakes, 0.56 - 0.7 mm
Capacity 2 mL
Certifications DNase and RNase Free
Format Tube; Filled
Hardness Zirconium sphere: Rockwell A 80-84, Vickers 1050, Garnet Flakes: Mohs 7.5-8
Applications Nucleic Acid Isolation, Nucleic Acid Extraction, Proteomics, Purification and Detection, Protein Purification, DNA Purification, Plant Tissue Processing, Tissue Analysis, Bacterial Lysis, Yeast Lysis, Fungal Tissue Analysis, Animal Cell Lysis, Animal Tissue Analysis, Microbiome Studies, Metabolite Extraction, Grind Seeds, Homogenization
Kit Compatibility DNA Kits, RNA Kits, Protein Purification Kits, FastDNA™ Kit, FastDNA™ Spin Kit, FastRNA™ Pro Kit, FastRNA™ Spin Kit, FastProtein Kit, GeneClean for Ancient DNA, GENECLEAN® Kit, all miniprep 2 mL kits, bead beating kits and any kit requiring processing of biological samples.
Material of Construction Matrix A contains garnet matrix and one 1/4 inch ceramic sphere in a Polypropylene Tube with a Cap.
Materials Required Not Supplied FastPrep® Instrument |Other High-speed Homogerizer |Vortexer |Centrifuge |Pipetors |Purification Reagents
Matrix Type A
Molecular Formula Banded Satellite: ZrO<sub>2</sub> 88%, CeO 10%, Garnet: Fe<sub>3</sub>Al<sub>2</sub>(SiO<sub>4</sub>)<sub>3</sub>
Molecule Enriched DNA |RNA |Proteins |Metabolites |Other small molecules
Personal Protective Equipment If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (labcoat and long-sleeves) and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices.
Physical Appearance 2 mL Screw Cap Tube with grinding particles installed
Presentation Box
Product Families Description Lysing Matrix is a critical component of any high-speed, reciprocating bead beating sample preparation operation. MP Biomedicals is the innovator in bead beating technology and developer of the FastPrep® Sample Preparation Systems. We manufacture the widest variety of Lysing Matrix products, optimized by sample type. Sample types include but are not limited to: human and animal and plant tissues; microorganisms like bacterial, yeast and fungi; plant, soil, fecal, plus insects and worms. MP Biomedicals receives matrix particles from proven vendors and ensures the unprocessed material meets high qualitative standards. Matrix particles are then prepared and dispensed under a rigorous set of proprietary conditions that offer customers a premium product that can be immediately used with confidence. Matrices are available separately for use with your own unique buffers, and are also available as components of the complete purification kits. Lysing Matrix is available in ready-to-use format in a variety of grinding vessels including tube sizes of 2 mL, 4.5 mL, 15 mL and 50 mL. High-throughput products include 96-Well Plates and 250 mL grinding bottles. All lysing matrix vessels are standard sizes to fit any homogenizer, centrifuge or vortexer on the market. Lysing Matrix products are available in bulk format for custom filling to your own specifications. For optimal performance and results we recommend using in conjunction with our FastPrep-24™ 5G Homogenizer and FastPrep® Extraction Kits. The FastPrep® System will easily grind, lyse and homogenize any sample type, from routine to the most resistant, in 40 seconds or less. Rapid, quantitative sample disruption results in the highest quality isolation of high MW DNA, stable RNA, biologically active proteins and other small molecules. |FastPrep® Lysing Matrix tubes are very versatile, consisting of 18 unique matrix compositions to successfully process any sample type! Whatever your sample, we have the solution. Our matrices contain properties that contribute to optimum success in your protocols. Various properties of a lysing matrix particle, whether inherent or selected, can yield a myriad of end-products depending on the dynamic qualities instilled in that particle by said properties. The inherent properties include the hardness, composition, and density, while the selected properties include the shape and the size. Here is a brief description of our properties: Size: The smaller the particles used in the grinding media, the smaller the average particle size and the smaller the lowest-limiting particle size produced during pulverization. (Note: this doesn’t mean that the smallest particles produced will be the same size as the smallest matrix particles used during lysis. The particles of the end product will always be much smaller that the smallest matrix particle. It’s rather like a bullet penetrating a glass window, producing pieces of shattered glass that are much smaller than the incident bullet.) Thus, matrix particle size should be selected based upon the size of the particles you wish to obtain in your lysate. For example, if your goal is to obtain intact and functional organelles, it’s best to utilize a larger grinding media. Utilizing a smaller size matrix would be more conducive to lysing smaller structures, such as viral particles and endospores. Shape: The shape of the grinding media is a major determining factor in the modality by which the cells are broken apart. Dull lysis media, such as those with spherical shapes, will complete a majority of the overall lysis by forces of cascade impaction, while the sharper and more jaggedly shaped lysis media will complete a majority of the overall lysis by shearing forces. Shearing forces are good for cracking open difficult-to-lyse samples, or samples that necessitate the isolation of a stable molecule such as DNA. RNA and certain easily denatured proteins are often vulnerable to shearing forces, while other molecules such as DNA, stable proteins (antibodies or collagen for instance), structural polysaccharides, and small molecules (such as small secondary metabolites) are immune to shearing forces, and thus it is practical to use shearing forces when isolating these, so as to eliminate those structures and functions that are vulnerable to such shearing forces. Hardness, Density and Composition: The composition determines two very important qualities: hardness and density, both of which are inherent properties derived from the molecular composition of the matrix particle. The hardness must be greater than that of the sample being pulverized. This is the case for all MP Biomedicals lysing matrices, so this variable is not as significant. The density is more important. The denser the matrix particles, the more effective they are at grinding up spores or tough tissues with dense extracellular matrices. It’s also important that the matrix particles being used are denser than the solution in which they are homogenizing, lest they simple float on top and fail to function in lysis or homogenization. Other qualities also accompany the matrix due to molecular structure. Some examples include electrical conductivity, melting point, and cross-reactivity, but these and other unmentioned inherent molecular properties are essentially insignificant due to our careful selection of the compositions of our lysing matrix particles. A Word About Knoop and Mohs: Materials chosen for use in FastPrep® Lysing Matrix products are carefully selected based on several criteria (discussed above). A key parameter is the hardness of the material, which can be evaluated by standard assays, which are reported for each of our quality reagents. The Knoop hardness test is a test for mechanical hardness of brittle materials or thin sheets. A small indentation is made in the surface using a diamond point applied with known force/time. The resulting indentation is measured using a microscope and scored with values typically from 100 to 1000, with increasing values being indicative of increase of material hardness. The Mohs scale defines the scratch resistance of various minerals, assessed as the ability of a harder material to scratch a softer material. Values typically range between 1 and 10, with diamond (for example having a Mohs scale score of 10).
Product Overview Each impact-resistant 2 mL tube contains garnet matrix and one 1/4 inch ceramic sphere. Extra 1/4 inch ceramic spheres are packaged separately. Lysing Matrix A tubes have orange caps and are found in the FastDNA™ and FastDNA™ SPIN Kits. Lysing Matrix A is used for all sample types except soil for the subsequent isolation of genomic DNA, RNA, functional proteins and other metabolites and small molecules. This grinding matrix is chemically inert and will not bind nucleic acids.
Research Areas Molecular Biology, Microbiology, Cell Biology, Biochemistry, Genomics, Gene Expression, Gene Regulation, Environmental and Ecology, Protein-Protein Interactions, Oncology, Transcription Regulation, Soil Science, Proteomics, Metagenomics, Metabolomics, Food Sciences, Forensics, Plant Pathology, Communicable Disease, Cancer, Agricultural, Microbiomics, Epigenetics
Sample Type Skeletal Muscle |Lung |Heart |Bone |Seeds |Tumor |Bacteria (Gram (+/-) |Yeast |Fungi |Spores |Plants |Fixed Samples |Sludge |Wastewater |Feces |Food |Roots |Soil |Animal Tissue |Tooth |Hair
Specific Gravity Zirconium sphere: 5.5 - 6.1 |Garnet Flakes: 4.0 - 4.1
Storage and Handling Store at Room Temperature (15-30 °C).
Tube Compatibility 2 mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.