Dino

FastDNA™ Spin Kit for Feces

FastDNA™ Spin Kit for Feces

Synonyms FastDNA™ Fecal Kit

Packaging size: 1 Kit

SKU 116570200
Price:
$312.25
In Stock
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Description

Product Description

The FastDNA SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

Host cells, bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds with any FastPrep® instrument.

The FastDNA SPIN Kit contains pre-filled 2 mL Lysing Matrix tubes for the homogenization step as well as all the reagents needed to extract DNA in a purification process eliminating PCR inhibitors.

Application Notes

The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used to isolate PCR-ready genomic DNA from fresh or frozen human and animal stool samples

Specifications
SKU 116570200
FastPrep Instruments Models QuickPrep (24x2 mL); HiPrep (48x2 mL); CoolPrep (24x2 mL); QuickFlex (96x2 mL); ConeFlex Legacy (allows all FP24 adapters on FP96)
Application Notes The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
Certifications DNase and RNase Free Lysing Matrix Tubes
Components Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each
Hardness Ceramic Spheres, Vickers 800; Silica Spheres, Knoop 515; Glass Bead, Knoop 515
Applications Used to isolate PCR-ready genomic DNA from fresh or frozen human and animal stool samples
Material of Construction Matrix E, 1.4 mm ceramic spheres, 0.1 mm silica spheres, and one 4 mm glass bead
Materials Required Not Supplied FastPrep® Instrument or alternative high speed disruptor, Microcentrifuge that can freely spin 2.0 mL tubes, Microcentrifuge tubes (2.0 mL and 1.5 mL), Sterile 15 mL tubes for DNA binding, Rotator or low-speed vortex, 100% Ethanol
Matrix Type E
Molecule Enriched DNA
Pack Size 1 Kit
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Reagent: Sodium Phosphate Buffer Sodium Phosphate Buffer- pH Range: 7.8-8.2, Reagent: MT Buffer MT Buffer pH Range: 5.8-6.2 , Reagent: PPS Protein Precipitate Solution pH Range: 5.8-6.2 , Reagent: Binding Matrix Binding Matrix pH Range: 6.5-7.2, Reage
Product Overview Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
Sample Type Soil; Feces; Environmental Water; Wastewater; Sludge
Sample Volume up to 500 mg per prep
Specific Gravity Ceramic Spheres, 4; Silica Spheres, 2.5; Glass Bead, 2.5
Storage and Handling All FastDNA® SPIN Kit reagents are shipped ambiently. FastDNA® SPIN Kit reagents are stable at room temperature. (15-30 °C).
Tube Compatibility 2 mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.