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FastDNA™ Spin Kit for Soil - 50 mL

FastDNA™ Spin Kit for Soil - 50 mL

Synonyms FastDNA Soil Kit

Packaging size: 1 Kit

SKU 116560600
Price:
$246.80
In Stock
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Description

Product Description

The FastDNA Spin Kit for Soil is designed to isolate bacterial, fungal, plant, and animal genomic DNA from soil and other environmental samples. Soil is processed by a FastPrep® instrument and Lysing Matrix E tubes designed to efficiently lyse all microorganisms. The released DNA is purified by a silica-based spin filter method and is suitable for PCR analysis and other downstream applications. Complete lysis of all organisms, including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi is achieved within seconds using a FastPrep instrument and Lysing Matrix E tubes.

Application Notes

Cited in over 6000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.

Specifications
SKU 116560600
FastPrep Instruments Models BigPrep (2x50 mL); CoolBigPrep (2x50 mL); BigFlex (8x50 mL)
Application Notes Cited in over 6000 scientific publications, the FastDNA Spin Kit for Soil delivers the highest DNA yield from any environmental sample including soil, sediments, sludge, compost, manure, rhizosphere or waste water. The FastDNA Spin Kit for Soil contains Lysing Matrix tubes and all the reagents needed to extract DNA in a process that efficiently removes humic acids and other PCR inhibitors
Brand FastDNA™
Certifications DNase and RNase free Lysing Matrix tubes
Components 10 x 50 mL Garnet Lysing Matrix Tubes, 60 mL PLS Solution, 15 mL MT Buffer, 350 mL Sodium Phosphate Buffer, 25 mL PPS for Soil, 120 mL Binding Matrix, 120 mL GTC Wash Buffer, 12 mL SEWS-M, 60 mL TES, 200 µL BBS Gel Loading Dye 200, 10 x 50 mL SPIN Filter Tubes
Hardness Mohs 7.5-8
Applications Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
Material of Construction Garnet Lysing Matrix
Materials Required Not Supplied FastPrep instrument to lyse samples, FastPrep appropriate 50 mL tubes adapter, Centrifuge that can freely spin 50 mL tubes, 50 mL centrifuge tubes, Sterile 15 mL tubes for DNA binding, Rotator
Matrix Type E
Molecule Enriched DNA
Pack Size 1 Kit
Personal Protective Equipment If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (Labcoat and long-sleeves), and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices.
Product Overview Up to 5 g of soil are processed by the FastPrep®-24 or FastPrep®-96 instrument with the Lysing Matrix E tubes designed to efficiently lyse all microorganisms including difficult sources such as eubacterial spores and endospores, gram positive bacteria and yeast. The released DNA is purified by a silica-based spin filter columns and removes humic acids/polyphenols that can inhibit PCR. The resulting pure DNA is suitable for PCR analysis and other downstream applications.
Research Areas Soil & Environmental Science
Sample Type Soil||Plants||Animal Tissues||Feces||Yeast||Bacteria (Gram +/-)||Fungi||Spores||Wastewater||Food||Seeds||Roots
Sample Volume Approximately 200 µL to 50 mL; up to approximately 25 g
Specific Gravity Garnet Flakes: 4.0 - 4.1
Storage and Handling All FastDNA Spin Kit for Soil reagents are shipped ambiently. FastDNA Spin Kit for Soil reagents are stable at room temperature. (15-30 ° C).
Tube Compatibility 50 mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.