PPS is a protein precipitation solution used in conjunction with a the CLS-VF lysing buffer for homogenization of plant tissue for the subsequent extraction and purification of nucliec acids.
PPS is component of FastDNA® SPIN Kit for Soil.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Protein precipitation solution to separate proteins from lysate during nucliec acid extraction
|FastPrep Instruments Models||All FastPrep Adapters|
|Alternate Names||Protein Precipitate Solution|
|Application Notes||PPS is component of FastDNA® SPIN Kit for Soil.|
|Boiling Point||100 °C (212 °F)(Lit.)|
|Clarity NTU||Colorless, precipitate-free solution|
|Density||0.8844 g/cm³ (7.38 lbs/gal) at 20 °C (68 °F)(Lit.)|
|Applications||Protein precipitation solution to separate proteins from lysate during nucliec acid extraction|
|Kit Compatibility||FastDNA and FastDNA Spin||FastRNAPro Soil Direct||FastRNAPro Soil Indirect|| FastDNA Spin Kit for Feces||FastDNA 50 mL Spin Kit for Soil||microprep <2 mL up to maxiprep 50 mL|
|Materials Required Not Supplied||FastPrep® Instrument or alternative high speed disruptor, Appropriate Lysing Matrix Tubes, Microcentrifuge, Pipettmen, Nucleic Acid Purification kit and/or Chloroform, 100% ethanol, 75% ethanol, 1.5 or 2.0 mL nuclease-free microcentrifuge tubes, Agarose, Gel loading dye and RNA size marker|
|Personal Protective Equipment||Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||Clear Liquid|
|Product Overview||PPS is a lysis solution component included in the FastDNA and the FastDNA Spin Kits. Used as a stabilization agent during sample lysis of primarily plant tissue, but can also be used for other sample types that are high in inhibitory compounds.|
|Sample Type||Plants||Animal Tissues||Fungi||Spores||Seeds||Roots|
|Sample Volume||up to 500 mg per prep|
|Storage and Handling||All Fast DNA Kit reagents are stable at room temperature(15-30 °C). Shelf life can be extended by storing at 4 °C.|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|
|Vapor Pressure||23 hPa (17 mm Hg) at 20 °C (68 °F)(Lit.)|