FASTRNA PRO SOIL-INDIRECT KIT

FASTRNA PRO SOIL-INDIRECT KIT

Packaging size: 2 mL

SKU 116075050
Price:
$468.40
In Stock
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Description

Product Description

Isolation of total RNA from soil supernatants The FastRNA™ Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by any FastPrep® Instrument in impact-resistant 2 mL tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX™ and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.

Application Notes

FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Isolation of RNA from soil samples

Specifications
SKU 116075050
FastPrep Instruments Models QuickPrep (24x2 mL)||HiPrep (48x2 mL)||CoolPrep (24x2 mL)||QuickFlex (96x2 mL)||ConeFlex Legacy (allows all FP24 adapters on FP96)
Application Notes FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications.
Components RNApro™ Solution, Lysing Matrix E, Inhibitor Removal Solution, DEPC-treated H2O, RNAMATRIX® Binding Solution, RNAMATRIX® Slurry, RNAMATRIX® Wash Solution, Quick-Clean Spin Filters
Applications Isolation of RNA from soil samples
Material of Construction Matrix E, 1.4 mm ceramic spheres, 0.1 mm silica spheres, and one 4 mm glass bead
Materials Required Not Supplied Resuspension Solution (either deionized H2O, 10 mM Tris [pH 7.0 - 8.0], or 100 mM NaCl), RNase EraseTM (Cat # 2440-204), recommended, FastPrep® Instrument or alternative high speed disruptor, 100% Ethanol, Chloroform, Centrifuge capable of spinning at
Matrix Type Lysing Mat
Molecule Enriched RNA
Pack Size 2 mL
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Research Areas OTHER - Drug Discovery|| OTHER - Soil & Environmental Science
Sample Type Soil||Feces||Environmental Water||Wastewater||Sludge
Sample Volume up to 500 mg per prep
Storage and Handling Store at Room Temperature (15-30 °C).
Trade Name FastRNA PRO™
Tube Compatibility 2 mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.