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FASTRNA SPIN KIT FOR YEAST

FASTRNA SPIN KIT FOR YEAST

Packaging size: 50 Preps

SKU 116030050
Price:
$320.10
In Stock
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Description

Product Description

The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialized Lysing Matrix (Yttria-stabilized zirconium oxide, 0.5mm) for cell lysis and SPIN columns for the purification process.

Application Notes

The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialized Lysing Matrix (Yttria-stabilized zirconium oxide, 0.5mm) for cell lysis and SPIN columns for the purification process.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used to isolate high-quality; total RNA from tough-to-lyse yeast strains; fungi; and algae.

Specifications
SKU 116030050
FastPrep Instruments Models QuickPrep (24x2 mL); HiPrep (48x2 mL); CoolPrep (24x2 mL); QuickFlex (96x2 mL); ConeFlex Legacy (allows all FP24 adapters on FP96)
Application Notes The FastRNA™ SPIN Kit for Yeast quickly and efficiently isolates high-quality, total RNA from tough-to-lyse yeast strains, fungi, and algae in approximately 15 minutes using a specialized Lysing Matrix (Yttria-stabilized zirconium oxide, 0.5mm) for cell lysis and SPIN columns for the purification process.
Components Lysing Matrix Y (0.5mm Yttria-stabilized ZrO2) 50 x 2-mL tubes, FastRNA Lysis Buffer 50 mL, FastRNA Prep Buffer 25 mL, FastRNA Wash Buffer 12 mL, Water (DNase/RNase-Free) 1 mL, FastRNA Spin Column 1 50 each, FastRNA Spin Column 2 50 each, Collection Tubes 2mL 100.
Applications Used to isolate high-quality; total RNA from tough-to-lyse yeast strains; fungi; and algae.
Material of Construction Matrix Y, 0.5 mm diameter Yttria-Stabilized Zirconium Oxide
Materials Required Not Supplied FastPrep® Instrument or alternative high speed disruptor, Microcentrifuge that can spin up to 14,000 rpm, 100% Ethanol (or 95% Ethanol)
Molecule Enriched RNA
Pack Size 50 Preps
Personal Protective Equipment Personal Protective Equipment: Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields. Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Product Overview Isolates both large and small RNA species without the use of phenol or reducing agents. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol. RNA (~5 µg) isolated from 101 to 105 cells can be eluted into volumes as little as 6 µl in less than 15 minutes. High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. RNA is eluted with RNase-free water and can be stored at =-70 °C. The addition of RNase inhibitors is optional, but highly recommended for prolonged storage.
Sample Type Yeast; Fungi; Spores; Algae
Storage and Handling All FastRNA™ SPIN Kit for Yeast reagents are shipped ambiently. FastRNA™ SPIN Kit for Yeast reagents are stable at room temperature. (15-30 °C).
Tube Compatibility 2 mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.