FASTRNA PROBLUE KIT UN2922-III
Product Description
The FastRNA® Pro Blue Kit is designed to efficiently isolate total RNA from gram positive and gram negative bacteria. Using the FastPrep®-24 or FastPrep® FP120 Instrument, up to 1010 cells are homogenized by Lysing Matrix B in impact-resistant 2 mL tubes. Total RNA is released into the proprietary, protective RNApro™ Solution.
Application Notes
FastRNA® Pro Blue Kit is used to isolate total RNA from gram positive and gram negative bacteria. Isolated RNA is suitable for RT-PCR analysis and other downstream applications.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Isolation of RNA from gram positive and gram negative bacteria
| SKU | 116025050 |
| FastPrep Instruments Models | QuickPrep (24x2 mL)||HiPrep (48x2 mL)||CoolPrep (24x2 mL)||QuickFlex (96x2 mL)||ConeFlex Legacy (allows all FP24 adapters on FP96) |
| Application Notes | FastRNA® Pro Blue Kit is used to isolate total RNA from gram positive and gram negative bacteria. Isolated RNA is suitable for RT-PCR analysis and other downstream applications. |
| Components | RNApro™ Solution 1 x 55 mL bottle||DEPC-H2O 1 x 15 mL bottle||Lysing Matrix B 50 x 2 mL tubes |
| Applications | Isolation of RNA from gram positive and gram negative bacteria |
| Material of Construction | Matrix B, 0.1mm zirconium silicate |
| Materials Required Not Supplied | FastPrep® Instrument or alternative high speed disruptor, Microcentrifuge, Pipettmen, RNase Erase® (Cat # 2440-204), recommended, Chloroform, 100% ethanol, 75% ethanol, 1.5 or 2.0 mL RNase-free microcentrifuge tubes, Agarose, Gel loading dye and RNA size marker |
| Matrix Type | Lysing Mat |
| Molecule Enriched | RNA |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Sample Type | Bacteria (Gram +/-) |
| Sample Volume | up to 500 mg per prep |
| Storage and Handling | Store at Room Temperature(15-30 °C). |
| Tube Compatibility | 2 mL |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |