FASTRNA SPIN KIT FOR MICROBES
Product Description
The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process.
Application Notes
The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Key Applications
Used to isolate high-quality; total RNA from tough-to-lyse bacterial cell cultures and other microbial sources .
| SKU | 116020050 |
| FastPrep Instruments Models | QuickPrep (24x2 mL); HiPrep (48x2 mL); CoolPrep (24x2 mL); QuickFlex (96x2 mL); ConeFlex Legacy (allows all FP24 adapters on FP96) |
| Application Notes | The FastRNA™ SPIN Kit for Microbes quickly and efficiently isolates high-quality, total RNA from tough-to-lyse bacterial cell cultures and other microbial sources in approximately 15 minutes using a zirconium silicate Lysing Matrix (0.1mm) to lyse bacteria and SPIN columns for the purification process. |
| Components | Lysing Matrix B (0.1mm zirconium silicate) 50 x 2-mL tubes, FastRNA Lysis Buffer 50 mL, FastRNA Prep Buffer 25 mL, FastRNA Wash Buffer 12 mL, Water (DNase/RNase-Free) 1 mL, FastRNA Spin Column 1 50 each, FastRNA Spin Column 2 50 each, Collection Tubes 2mL 100 each. |
| Applications | Used to isolate high-quality; total RNA from tough-to-lyse bacterial cell cultures and other microbial sources . |
| Material of Construction | Matrix B, 0.1mm zirconium silicate |
| Materials Required Not Supplied | FastPrep® Instrument or alternative high speed disruptor, Microcentrifuge that can spin up to 14,000 rpm, 100% Ethanol (or 95% Ethanol) |
| Molecule Enriched | RNA |
| Personal Protective Equipment | Personal Protective Equipment: Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields. Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Product Overview | Isolates both large and small RNA species without the use of phenol or reducing agents. Small RNAs (e.g., tRNAs, microRNAs) can be recovered following a simple adjustment within the RNA isolation protocol. RNA (~5 µg) isolated from 101 to 105 cells can be eluted into volumes as little as 6 µl in less than 15 minutes. High quality total RNA (A260/A280 > 1.8, A260/A230 > 1.8) is recovered. RNA is eluted with RNase-free water and can be stored at =-70 °C. The addition of RNase inhibitors is optional, but highly recommended for prolonged storage. |
| Sample Type | Bacteria (Gram +/-) |
| Storage and Handling | All FastRNA™ SPIN Kit for Microbes reagents are shipped ambiently. FastRNA™ SPIN Kit for Microbes reagents are stable at room temperature. (15-30 °C). |
| Tube Compatibility | 2 mL |
| Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |