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SDA MEDIUM-LEU (PACK)(POUCHES)

SDA MEDIUM-LEU (PACK)(POUCHES)

Synonyms synthetic defined medium without leucine

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SKU 114811165-CF
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$169.05
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Description

Product Description

Single dropout formulation (without Leucine) of Synthetic Defined (SDA) Yeast Media with agar includes all necessary components for selective growth of Saccharomyces cerevisiae.

Application Notes

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Specifications
SKU 114811165-CF
Application Notes The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Cells Tested Saccharomyces cerevisiae
Certifications Molecular Biology Certified™
Clarity NTU Clear to Slightly Hazy
Color APHA Colorless to Faint Yellow
Media Type Yeast Media
Format powder
Formulation Details Contents per Liter: 1.7 g YNB |5 g Ammonium Sulfate |20 g Dextrose |0.69 g CSM-Leu |17 g Agar-Y
Grade Molecular Biology Certified™
Applications Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
Kit Compatibility Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
pH pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C
Physical Appearance Powder
Preparation Method Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Synthetic Defined (SD) or Synthetic Defined Agar (SDA) or SD/SDA-Dropout formula. SD is 1.7 g Yeast Nitrogen Base, 5 g Ammonium Sulfate and 20g Dextrose per liter. SDA is SD with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium by adding 27.49 SD powder or the appropriate weight ofSD-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes.
Presentation Box
Product Overview Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of dropouts for flexibile selectivity.
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation
Solubility Soluble in water.
Sterilization of Solutions Autoclave at 121 °C for 15 minutes.
Storage and Handling Store dry powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.