6 dropout formulation (without Adenine, Histidine, Leucine, Lysine, Tryptophan and Uracil) of Complete Supplement Mixture (CSM) of amino acids for S. cerevisiae growth media. Time tested formulation is available in complete and dropout formulations.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Complete Supplement Mixture|
|Application Notes||The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Clarity NTU||Clear to Slightly Hazy|
|Color APHA||Colorless to Faint Yellow|
|Media Type||Yeast Media|
|Formulation||Contents per Liter: Adenine 0* mg |L-Arginine HCl 50 mg |L-Aspartic Acid 80 mg |L-Histidine HCl 0 mg |L-Isoleucine 50 mg |L-Leucine 0 mg |L-Lysine HCl 0mg |L-Methionine 20** mg |L-Phenylalanine 50 mg |L-Threonine 100** mg |L-Tryptophan 0 mg |Uracil 0 mg| L-Tyrosine 50 mg |L-Valine 140 mg |Total 540.000 |*Minimum quantity for healthy growth and yet optimized to promote red color in certain adenine auxotrophs. CSM formulations are available that contain 20 or 40 mg/liter adenine. **80mg/liter of DL-Homoserine is substituted for L-Threonine in mixtures where L-Methionine is dropped out.|
|Grade||Molecular Biology Certified™|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the DOB or DOBA with CSM or CSM-Dropout formula. DOB (Drop Out Base) is 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, 20g Dextrose per liter. DOBA is DOB with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid plate medium by adding 43.7g DOBA powder and 0.79g of CSM or the appropriate weight of CSM-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. After cooling to 50 °C plates were poured.|
|Product Overview||For use with DOB/DOBA or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in water.|
|Sterilization of Solutions||Autoclave at 121 °C for 15 minutes|
|Storage and Handling||Store dry powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|