Double dropout formulation (without L-Leucine and Uracil) of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Also known as Hopkins Mixture and is available in complete and dropout formulations.
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This SC-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Synthetic Complete Supplement Mixture; Hopkins Mixture|
|Application Notes||The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This SC-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Clarity NTU||Clear to Slightly Hazy|
|Color APHA||Colorless to Faint Yellow|
|Media Type||Yeast Media|
|Formulation Details||Contents per Liter: Adenine 21 mg; L-Alanine 86.5 mg; L-Arginine HCl 85.6 mg; L-Asparagine 85.6 mg; L-Aspartic Acid 85.6 mg; L-Cysteine HCl 85.6 mg; Glutamine 85.6 mg; L-Glutamic Acid 85.6 mg; Glycine 85.6 mg; L-Histidine HCl 85.6 mg; Myo-Inositol 85.6 mg; L-Isoleucine 85.6 mg; L-Leucine 0 mg; L-Lysine HCl 85.6 mg; L-Methionine 85.6 mg; Para-Aminobenzoic Acid 8.6 mg; L-Phenylalanine 85.6 mg; L-Proline 85.6 mg; L-Serine 85.6 mg; L-Threonine 85.6 mg; L-Tryptophan 85.6 mg; L-Tyrosine 85.6 mg; Uracil 0 mg; L-Valine 85.6 mg; Total 2000.6 mg|
|Grade||Molecular Biology Certified™|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Materials Required Not Supplied||YNB+carbon source and Agar if needed and DOB or DOBA|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Drop Out Base (DOB) or Drop Out Base with 17 g Agar (DOBA) formula, with SC or SC-dropout formulations. DOB contains 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, and 20g Dextrose per liter. Chosen reagents are combined in the proper proportions according to the formulation listed on the Certificate of Analysis and milled to thoroughly blend the reagents for a uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium by adding 43.7g DOBA powder and 2 g of SC to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 15 minutes. After cooling to 50 °C, plates were poured. DOBA with SC liquid solution was visually inspected after autoclaving and pouring plates for complete dissolution of components. Solid plates were inoculated with control S.cerevisiae yeast strain and incubated for 3 days at 30 °C. Colony size was compared to the same strain grown on standard lot test media (SLTM).|
|Product Overview||For use with DOB/DOBA or YNB with the appropriate nitrogen and carbon sources to custom formulate your own selective Synthetic Defined yeast media|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in water.|
|Sterilization of Solutions||Autoclave at 121 °C for 15 minutes|
|Storage and Handling||Store dry powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|