EMM NO NITROGEN (NO NHCL, G
EMM Nitrogen (No NH4Cl, Glutamate) is a minimal defined medium for the growth of S. pombe, containing dextrose, buffers, salts, vitamins and trace minerals. EMM minimal defined medium is thiamine-free nmt1 promoter studies in Schizosaccharomyces pombe.
EMM w/o Nitrogen is used for the growth of S.pombe.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
|Alternate Names||Edinburgh's Minimal Medium No Nitrogen |S.pombe growth medium|
|Application Notes||EMM w/o Nitrogen is used for the growth of S.pombe.|
|Cells Tested||Schizosaccharomyces pombe|
|Certifications||Certified Thiamine (vitamin B1) free|
|Media Type||Yeast Media|
|Formulation||Contents Per Liter: 20 g d-glucose; 3 g potassium hydrogen phthalate (14.7 mM); 2.2 g Na2HPO4 (15.5 mM); 1.05 g MgCl2.6H2O (5.2 mM); 14.7 µg CaCl2.2H2O (99.8 µM); 1 g KCl (13.4 mM); 0.04 g Na2SO4 (0.28 mM); 1mg pantothenic acid (4.2 µM); 10 mg nicotinic acid (81.2 µM); 10 mg myo-inositol (55.5 µM); 10 µg biotin (0.82 µM); 0.5 mg boric acid (8.9 µM); 0.4 mg MnSO4 (2.4 µM); 0.4 mg ZnSO4.7H2O (1.4 µM); 0.2 mg FeCl2.6H2O (0.7 µM); 40 µg molybdic acid (0.25 µM); 0.1 mg KI (0.6 µM); 40 µg CuSO4.5H2O (0.016 µM); 1 mg citric acid (4.76 µM).|
|Grade||Molecular Biology Certified™|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range: 5.4 - 6.4 at 25 °C|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in EMM Broth formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Product Overview||EMM w/o Nitrogen (NH4Cl) w/o Glutamate is a minimal defined media used for the growth of Schizosaccharomyces pombe. Use for studies that require nitrogen-free growth conditions.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in water (27 g/L).|
|Sterilization of Solutions||Autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).|
|Storage and Handling||Store dry powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|