YES is a nutritionally rich medium for the growth of S. pombe, containing dextrose, and yeast extract. Dextrose is the energy source. Yeast extract provides carbon, nitrogen, minerals, vitamins, trace ingredients and other essential growth nutrients which stimulate the growth of yeasts.
EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture
|Application Notes||EMM (Edinburgh Minimal Medium) is used for the growth of fission yeast S. Pombe.|
|Cells Tested||Schizosaccharomyces pombe|
|Certifications||Certified Thiamine (vitamin B1) free|
|Media Type||Yeast Media|
|Formulation Details||Contents Per Liter: 5 g Yeast Extract-Y; 30 g Dextrose; 1X Supplements (50 mg each ADE; HIS; LEU; LYS; URA). Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. pombe Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||pH Range: 5.5 - 6.5 at 25 °C|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. pombe when combined in YES Broth formulations. Chosen reagents are combined in the proper proportions according to the YES formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Product Families Description||Schizosaccharomyces pombe has different nutritional needs than S.cerevisiae. MP Biomedicals offers these special growth and minimal defined media formulations specific to the needs of S. pombe. All S. pombe media is certified thiamine-free for nmt1 promoter studies. Our wide range of yeast nitrogen bases, sugars, and amino acid dropout mixtures can be mixed together to form almost any complete media imaginable. Many of these combinations are already available off-the-shelf as pre-mixed powders. All media and media components are quantitatively and qualitatively tested before their release to guarantee the growth of healthy, dense cultures. |Choices in formulation include with, or without agar, and packaging choices are: |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!|
|Product Overview||YPD Growth medium is a standard complete formulation for the nonselective growth of rich, fully confluent S. pombe cultures.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in water (35 g/L).|
|Storage and Handling||Store dry powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|