}{ 5-FOA (POWDER)

}{ 5-FOA (POWDER)

Synonyms 2,6-Dihydroxy-5-fluoropyrimidine-4-carboxylic acid; 5-Fluorouracil-4-carboxylic acid; 5-Fluoroorotate; Fluoroorotic Acid; FOA; 4-Pyrimidinecarboxylic Acid; 5-Fluoro-1,2,3,6-Tetrahydro-2,6-Dioxo-(9CI); 1,2,3,6-Tetrahydro-2,6-Dioxo-5-Fluoro-4-Pyrimidinecarboxylic Acid; 5-Fluoro-6-Carboxyuracil

CAS Number: 703-95-7 Molecular Formula: C5H3FN2O4·H2O Molecular Weight: 174.09 (anhydrous)

EC Number: 211-876-0

Packaging size: 1 g

SKU 114066102
Price:
$451.95
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Description

Product Description

5-Fluoroorotic Acid monohydrate is a derivative of a pyrimidine precursor and is selectively toxic to yeast cells which synthesize orotidine-5’-phosphate decarboxylase.

Application Notes

5-Fluoroorotic Acid monohydrate (FOA) is used as a selective agent in yeast molecular genetics. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. It is also used in the selection of Ura cells in a population of Ura+ cells; and in the selection of orotidine-5’-phosphate decarboxylase (OMPdecase) mutants of Saccharomyces cerevisiae. FOA (0.1 mg/mL) has been used in the positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius. It is a noncompetitive inhibitor of dihydroorotase. FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in vitro and in vivo. FOA showed anti-tumor activity against transplanted tumors in rats and mice; a bacteriostatic effect in vitro against various microorganisms, particularly gram-negative bacteria; antimycotic activity against various types of mold.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Specifications
SKU 114066102
Application Notes 5-Fluoroorotic Acid monohydrate (FOA) is used as a selective agent in yeast molecular genetics. Curing yeast strains of plasmids, plasmid shuffling, allelic replacement, and two-hybrid screens are methods that can employ the use of 5-FOA. It is also used in the selection of Ura cells in a population of Ura+ cells; and in the selection of orotidine-5’-phosphate decarboxylase (OMPdecase) mutants of Saccharomyces cerevisiae. FOA (0.1 mg/mL) has been used in the positive selection for uracil auxotrophs of the sulfur-dependent thermophilic archaebacterium Sulfolobus acidocaldarius. It is a noncompetitive inhibitor of dihydroorotase. FOA (50% inhibition, 6 nM) was a selective inhibitor of malarial cells of Plasmodium falciparum in vitro and in vivo. FOA showed anti-tumor activity against transplanted tumors in rats and mice; a bacteriostatic effect in vitro against various microorganisms, particularly gram-negative bacteria; antimycotic activity against various types of mold.
Cells Tested Saccharomyces cerevisiae
Certifications Molecular Biology Certified™
Media Type Yeast Media
EC Number 211-876-0
Format powder
Grade Molecular Biology Certified™
Applications Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
Melting Point 278 °C (dec.) (Lit.)
Molecular Formula C5H3FN2O4·H2O
Molecular Weight 174.09 (anhydrous)
Pack Size 1 g
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
Physical Appearance Powder
Presentation Bottle
Product Overview Add to media when working with yeast to select for Ura-. Ura+ cells will not grow in the presence of 1 mg/mL 5-FOA.
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation
Safety Symbol GHS07
Solubility Soluble in 4 M NH4OH (50 mg/mL)(Sonication or heating may be needed), slightly soluble in water.
Storage and Handling Store at -0 °C.
Typical Working Concentration 1 mg/mL has been used as a selective agent in yeast molecular genetics
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.