X-GAL (POWDER) (m)

X-GAL (POWDER) (m)

Synonyms 5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG

CAS Number: 7240-90-6 Molecular Formula: C14H15BrClNO6 Molecular Weight: 408.6

Beilstein Registry Number: 1402009 EC Number: 230-640-8

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SKU 114063102-CF
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$148.85
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Description

Product Description

X-Gal (5-Bromo-4-Chloro-3-Indolyl-ß-D-galactopyranoside) is a β-galactosidase substrate. In the presence of β-galactosidase, X-Gal is hydrolyzed and yields a blue precipitate.

Application Notes

X-Gal is used for the identification and selection of galactosidase producing bacteria (bacteria Lac+). X-Gal is particularly useful in molecular biology applications to detect the activity of β-galactosidase which is frequently used as a reporter gene. In cloning, X-Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Lac? bacterial colonies resulting from α-complementation will appear blue whereas bacterial colonies containing plasmid with DNA inserted in the lacZ region, will be incapable of α-complementation and will appear white. Many other applications also use X-Gal as a substrate to detect β-galactosidase activity, which include β-galactosidase-antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction, and the detection of micrometastasis formation during tumor progession.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture

Specifications
SKU 114063102-CF
Application Notes X-Gal is used for the identification and selection of galactosidase producing bacteria (bacteria Lac+). X-Gal is particularly useful in molecular biology applications to detect the activity of β-galactosidase which is frequently used as a reporter gene. In cloning, X-Gal is used to detect insertion of foreign DNA into the lacZ region of plasmid DNA using α complementation which is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β-galactosidase. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Lac? bacterial colonies resulting from α-complementation will appear blue whereas bacterial colonies containing plasmid with DNA inserted in the lacZ region, will be incapable of α-complementation and will appear white. Many other applications also use X-Gal as a substrate to detect β-galactosidase activity, which include β-galactosidase-antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction, and the detection of micrometastasis formation during tumor progession.
Beilstein Registry Number 1402009
Cells Tested Saccharomyces cerevisiae
Certifications Molecular Biology Certified™
Media Type Yeast Media
Detection Method FTIR
EC Number 230-640-8
Format powder
Grade Ultra pure
Applications Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
Molecular Formula C14H15BrClNO6
Molecular Weight 408.6
Optical Rotation -61.9%(c=1, DMF:water=1:1, Lit.)
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice
Physical Appearance Powder
Presentation Bottle
Product Families Description MP Biomedicals offers high quality components for preparation of your own custom formulations for growth and maintenance of Yeasts. Peptones, yeast extracts, carbon sources, amino acids, salts, trace minerals, agars and other reagents are available. Choices in formulation allow for custom formulations from small research batches up to pilot quantities. |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!
Product Overview X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background.
Purity (TLC) >99%
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation
Solubility Soluble in dimethylformamide (2% in DMF), Dimethyl sulfoxide (20 mg/mL) & DMF:MeOH (1:1) (50 mg/mL).
Storage and Handling Store at -20°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.