YNB W/ AMMONIUM SULFATE, W/O I
YNB is available with or without ammonium sulfate. YNB is composed of salts, vitamins and trace elements. Some component dropouts are available. To make your own synthetic defined yeast media, start with the YNB of your choice, add a carbon source, the appropriate amino acids, and agar if necessary.
YNB w/ Ammonium Sulfate w/o Inositol is a component of synthetic defined yeast media.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Yeast Nitrogen Base|
|Application Notes||YNB w/ Ammonium Sulfate w/o Inositol is a component of synthetic defined yeast media.|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Media Type||Yeast Media|
|Formulation||Components Per liter medium-Nitrogen Sources: Vitamins: Biotin 0.002 mg; Calcium Pantothenate 0.4 mg; Folic Acid 0.002 mg; Niacin 0.4 mg; P-Aminobenzoic Acid 0.2 mg; Pyridoxine Hydrochloride 0.4 mg; Riboflavin 0.2 mg; Thiamine Hydrochloride 0.4 mg; Trace Elements: Boric Acid 0.5 mg; Copper Sulfate 0.04 mg; Potassium Iodide 0.1 mg; Ferric Chloride 0.2 mg; Manganese Sulfate 0.4 mg; Sodium Molybdate 0.2 mg; Zinc Sulfate 0.4 mg; Salts: Magnesium Sulfate 500 mg; Sodium Chloride 100 mg; Potassium Phosphate; monobasic 1000 mg; Calcium Chloride 100 mg.|
|Grade||Molecular Biology Certified™|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||4.4 ± 0.8|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YNB formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution.|
|Product Overview||YNB without ammonium sulfate, w/o Copper Sulfate, w/o Ferric Chloride is a well defined composition of salts, vitamins and trace elements for a vigorous growth of S. cerevisiae.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in water (6.7 g/L)|
|Storage and Handling||Store dry YNB powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Typical Working Concentration||6.7g/liter|