DOB allows for preparation of a custom synthetic defined yeast media and includes a complete yeast nitrogen base (YNB) composed of buffering salts, vitamins and trace elements, with a carbon source.
DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Alternate Names||Dropout Base Medium |S.cerevisiae Growth medium|
|Application Notes||DOB is a component of Synthetic Defined Media (SD), which is used for growth of S. cerevisiae.|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Media Type||Yeast Media|
|Formulation Details||Contents/liter: 1.7 g YNB; 5g Ammonium Sulfate; 20 g Dextrose|
|Grade||Molecular Biology Certified™|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||5.0 ± 0.2|
|Preparation Method||Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in DOB formulations. Chosen reagents are combined in the proper proportions according to the formulation listed above and thoroughly blended for a uniform distribution.|
|Product Families Description||Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of formats for flexibility and convenience. MP Biomedicals offers many amino acid dropout media through combinations of our Complete Supplement Mixture (CSM), several carbon sources, and variations of YNB. If you need amino acid ratios different from those found in our CSM mixtures, we also offer classic amino acid formulations Hollenberg Supplement Mixture (HSM), Brent Supplement Mixture (BSM) and Synthetic Complete/Hopkins Mixture (SC). Synthetic defined media can be ordered in a number of ways: - Order a complete SD or SDA - Order a dropout Base (DOB) or Dropout Base with Agar (DOBA) plus the appropriate amino acid supplement mixture (CSM) - Order a Yeast Nitrogen Base (YNB), a carbon source, the appropriate amino acid supplement mixture (CSM), and agar if desired. - Order all raw materials separately Synthetic Defined Yeast Media: Formulas To Grow By! Synthetic defined yeast media includes all necessary components for selective growth of Saccharomyces cerevisiae. MP Biomedicals offers synthetic defined broth (SD) and synthetic defined agar (SDA) in a variety of formats for flexibility and convenience. Use the following table to navigate through the world of yeast media choices! | SDA = SD recipe + 17 g/L of Agar; DOBA = DOB recipe + 17 g/L of Agar |Use Yeast Nitrogen Base (YNB) Formulations to make your own custom synthetic defined yeast media. Start with the YNB of your choice, add a carbon source, the appropriate amino acids, and agar if necessary. |Dropout Base (DOB) and Dropout Base with Agar (DOBA) is a complete yeast nitrogen base with a carbon source. A complete supplemented synthetic defined medium is easily made by mixing DOB and Complete Supplement Mixture (CSM) which contains amino acids, adenine and uracil. CSM is also availble in Dropout formulations.|Packaging options include: Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Pre-poured Plates with agar, Ready-to-Use! |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!|
|Product Overview||DOB is a complete yeast nitrogen base with a carbon source. A complete supplemented synthetic defined medium is easily made by Mix DOB and CSM, a complete supplement mixture containing amino acids, adenine and uracil, of your own amino acid formulation to make a custom synthetic defined yeast media.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology |Transcription Regulation|
|Solubility||Soluble in Water (26.7 g/L)|
|Storage and Handling||Store dry DOB Medium powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Typical Working Concentration||26.7g/liter|