YPD (YEPD) Broth (Pack Pouches)
YPD (YEPD) is a nutritionally rich medium for the growth of S. cerevisiae, containing dextrose, peptone and yeast extract. Dextrose is the energy source. Yeast extract and peptone provide carbon, nitrogen, minerals, vitamins, trace ingredients and other essential growth nutrients which stimulate the growth of yeasts.
YPD Broth is used for routine growth of Saccharomyces cerevisiae for molecular biology studies.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
|Application Notes||YPD Broth is used for routine growth of Saccharomyces cerevisiae for molecular biology studies.|
|Cells Tested||Saccharomyces cerevisiae|
|Certifications||Molecular Biology Certified™|
|Media Type||Yeast Media|
|Formulation Details||Contents Per Liter: 20 g Peptone Y; 10 g Yeast Extract-Y; 20 g Dextrose. Small quantities of biological buffers have been added for proper pH adjustment.|
|Grade||Molecular Biology Certified™ Yeast Growth Medium|
|Applications||Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture|
|Kit Compatibility||Yeast RPM™ Kit |EZ-Yeast Transformation Kit |Alkali-Cation™ Yeast Transformation Kit |Yeast Cell Lysis Kit | FastDNA™ SPIN Kit |FastRNA™ Pro Red Kit |FastProtein™ Red Kit |FastRNA™ Spin Kit for Microbes |FastDNA™-96 Fungal/Bacterial DNA Kit|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice|
|pH||Acceptable pH range is 6.2 ± 0.3 at 25 °C|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YPD Broth formulations. Chosen reagents are combined in the proper proportions according to the YPD formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.|
|Product Families Description||MP Biomedicals offers an exceptional variety of quality yeast media. Our years of expertise helps you avoid the confusion of choosing the right media to selectively grow your yeast cells! Our wide range of yeast nitrogen bases, sugars, and amino acid dropout mixtures can be mixed together to form almost any complete media imaginable. Many of these combinations are already available off-the-shelf as pre-mixed powders. Both selective media and general growth media are available for S.cerevisiae and S. pombe, and all recipes are quantitatively and qualitatively tested before their release. Our Standard Growth Media for S. cerevisiae are extract based, complete media formulations for the growth of healthy, dense cultures. |Choices in formulation include with, or without agar, and packaging choices are: |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!|
|Product Overview||YPD Growth medium is a standard complete formulation for the nonselective growth of rich, fully confluent S. cerevisiae cultures.|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Protein-Protein Interactions |Oncology|
|Solubility||Soluble in water (25 g/0.5 L).|
|Sterilization of Solutions||Combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes. Note: Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not normally affect growth of cells, only the appearance of the sterile medium).|
|Storage and Handling||Store dry YPD Broth powder at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|
|Typical Working Concentration||50 g/L|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|