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TERRIFIC BROTH PART 1 (CAPSULE

TERRIFIC BROTH PART 1 (CAPSULE

Synonyms TB medium

Packaging size: 1 kg

SKU 113045031
Price:
$326.20
In Stock
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Description

Product Description

Terrific Broth is a classic rich medium for high density growth of E. Coli cells and higher yields of plasmid DNA without the need of antibiotic amplification. Similar in functin to CIRCLEGROW™ except Terrific Broth must be autoclaved in two parts with are combined after autoclaving.

Application Notes

Terrific Broth is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages

Specifications
SKU 113045031
Application Notes Terrific Broth is a rich nutrient media for propagation of Escherichia coli for purposes of strain maintenance, cloning, protein expression. It is certified for plasmid and other bacterial cultures in liquid medium. Higher cell densities are achieved using Terrific Broth in comparison to traditional LB Broth.
Cells Tested E. coli
Certifications Molecular Biology Certified™; Gelatin capsules are produced in a facility that meets the FDA’s recent guidance as being derived from BSE-free herds that are born, raised, and slaughtered in the United States.
Media Type Bacterial Media
Format Part 1:Capsules & Part 2:Solution
Formulation Details Contents Per Liter: Powder; Part 1: 24g Yeast Extract; 12g Tryptone. Liquid; Part 2: Potassium phosphate salts and glycerol in sterile solution.
Grade Molecular Biology Certified Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of M13 phage & other filamentous ssDNA bacteriophages
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Pack Size 1 kg
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.84±0.3 at 25 °C. Please note that the pH of Terrific Broth Medium liquid increases as the temperature of the solution decreases.
Physical Appearance Part 1:Capsules & Part 2:Solution
Preparation Method Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in Terrific Broth formulations. Chosen reagents are combined in the accurate proportions according to the formula and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid. The combinations were mixed to dissolve components and autoclaved at 121 °C for 15 minutes.
Presentation Jar and Bottle
Product Overview Terrific Broth consists of two separate parts. Part 1: 36 grams (36 capsules) per liter Contents/liter: 24 g yeast extract, 12 g tryptone Part 2: Potassium phosphate salts and glycerol in sterile solution
Recommended Replacement For CIRCLEGROW™
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Oncology
Solubility Soluble in water (36 g/L).
Sterilization of Solutions Autoclave part 1 at 121° C for 15 minutes. When cool, add 20 mL of sterile Part 2.
Storage and Handling Store dry Terrific Broth Powder Medium at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.