SOB (POWDER)

SOB (POWDER)

Synonyms Competent Cell Media for Bacteria

SKU 113030022
Price:
$62.50
In Stock
Request a quote
Description

Product Description

SOB media is a standard media the preparation of cells for chemical transformation.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Medium used in preparing Escherichia coli competent cells for transformation

Specifications
SKU 113030022
Alternate Names Competent Cell Media for Bacteria
Cells Tested E. coli
Certifications Molecular Biology Certified™
Media Type Bacterial Media
Format powder
Formulation Details Typically contains per liter: 20 g Tryptone-B; 5 g Yeast Extract-B; 0.5 g NaCl; 5 g MgSO4∙7H2O. Small quantities of biological buffers have been added for proper pH adjustment.
Grade Molecular Biology Certified™ Bacterial Growth Medium
Applications Medium used in preparing Escherichia coli competent cells for transformation
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.4-7.5 at 25 °C. Please note that the pH of SOB Medium liquid increases as the temperature of the solution decreases.
Physical Appearance Powder
Preparation Method Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in SOB formulations. Chosen reagents are combined in the proper proportions according to the SOB formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 31 g of powder per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121°C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).
Presentation Jar
Product Overview SOB media is for the preparation of competent host cells prior to transformation.
Recommended Replacement For 0910119 | 091011917
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology |Oncology
Solubility Soluble in water (31 g/L).
Storage and Handling Store dry SOB Powder Medium at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.