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SUPERBROTH (LARGE CAPSULES)

SUPERBROTH (LARGE CAPSULES)

Synonyms Bacteriophage Media

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SKU 113010036-CF
Price:
$372.10
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Description

Product Description

SuperBroth is enriched medium with high levels of tryptone and yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained.

Application Notes

Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of Lambda phage

Specifications
SKU 113010036-CF
Application Notes Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.
Boiling Point 1461 °C (2662 °F)(Lit.)
Cells Tested E. coli
Certifications Molecular Biology Certified™. Gelatin capsules are produced in a facility that meets the FDA’s recent guidance as being derived from BSE-free herds that are born, raised, and slaughtered in the United States.
Components 35 g tryptone, 20 g yeast extract, 5 g NaCl
Media Type Bacterial Media
Format Large Capsules
Formulation Details Contents Per Liter: 35 g Tryptone-B; 20 g Yeast Extract; 5 g NaCI.
Grade Molecular Biology Certified™ Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of Lambda phage
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.6 ± 0.2 at 25°C.
Physical Appearance Large capsules
Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in SuperBroth formulations. Chosen reagents are combined in the proper proportions according to the Superbroth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 60 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).
Presentation Jar
Product Families Description Bacteriophage manipulation is easier with the right media. MP Biomedicals offers media specifically designed for bacteriophage propagation, maintenance and manipulation. Quality components such as Tryptone-B, Yeast extract, NZ-amine, amino acids and sodium or magnesium salts are added in specialty formulations for Lambda phage, M13 phage and other filamentous ssDNA bacteriophages. MP Bio also offers formulations for competent cell preparation of phage containing lines. Other choices in formulation include with, or without agar, and packaging choices are: |Bulk powder, easily weighed to make custom batch sizes. |Pre-measured, sealed pouches, for 0.5 L or 1 L batch sizes, simply tear open the pouch and add to water. |Encapsulated, the Ultimate in Convenience. Simply count out the appropriate number of capsules and drop them in water!
Product Overview SuperBroth has the composition of 35 g tryptone-B, 20 g yeast extract, 5 g NaCl
Recommended Replacement For L-Broth
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology
Solubility Soluble in water (60 g/L).
Storage and Handling Store dry SuperBroth Powder Medium at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.