Take 10% off your next web order for ALL Life Science products with promo code: SHOPWEB10 Offer ends September 1st, 2020.
Super broth, large capsules
SuperBroth is enriched medium with high levels of tryptone and yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained.
Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of Lambda phage
|Alternate Names||Bacteriophage Media|
|Application Notes||Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.|
|Base Catalog Number||3010036|
|Boiling Point||1461 °C (2662 °F)(Lit.)|
|Components||35 g tryptone, 20 g yeast extract, 5 g NaCl|
|Formulation Details||Contents Per Liter: 35 g Tryptone-B; 20 g Yeast Extract; 5 g NaCI.|
|Grade||Molecular Biology Certified™|
|Media Type||Super Broth|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.6 ± 0.2 at 25°C.|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in SuperBroth formulations. Chosen reagents are combined in the proper proportions according to the Superbroth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 60 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Solubility||Soluble in water (60 g/L).|
|Usage Statement||Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.|