NZY-HARVARD BROTH (POWDER)
NZ formulations are for propagation of bacteriophage lambda.
NZY-Harvard broth is used for obtaining high yields of lambda phage and phagemids in E. coli.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of Lambda phage
|Alternate Names||Bacteriophage Media|
|Application Notes||NZY-Harvard broth is used for obtaining high yields of lambda phage and phagemids in E. coli.|
|Cells Tested||E. coli|
|Certifications||Molecular Biology Certified™|
|Media Type||Bacterial Media|
|Formulation Details||Contents Per Liter: 8 g NaCl; 10 g NZ Amine; 5 g yeast extract|
|Grade||Molecular Biology Certified™ Bacterial Growth Medium|
|Applications||Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation |Growth and Maintenance of Lambda phage|
|Kit Compatibility||Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines|
|Pack Size||454 g|
|Personal Protective Equipment||Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|pH||Acceptable pH range is 6.6 ± 0.2 at 25°C.|
|Preparation Method||Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in NZY-Harvard Broth formulations. Chosen reagents are combined in the proper proportions according to the NZY-Harvard Broth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 23 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).|
|Product Overview||NZY-(Harvard) Broth has the composition of 8 g NaCl, 10 g NZ Amine, 5 g yeast extract|
|Recommended Replacement For||SuperBroth|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology|
|Solubility||Soluble in water (23 g/L).|
|Storage and Handling||Store dry NZY Harvard Medium at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.|