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LB AGAR MEDIUM (STERILE SOLUTION)

LB AGAR MEDIUM (STERILE SOLUTION)

Synonyms Luria Bertani Medium with Agar |Luria Bertani Broth with Agar |Luria Broth Base Miller Formulation with Agar

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SKU 113002204-CF
Price:
$35.80
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Description

Product Description

LB-Agar medium is the most widely used medium for the growth of bacteria. MP Biomedicals offers LB media in a variety of formats for convenience and ease of use. It is a rich Medium for the growth of E. coli and certified for plasmid and other bacterial cultures in liquid medium. Agar is already included in this convenient formulation. MP Biomedicals offers LB media in an encapsulated format for convenience and ease of use.

Application Notes

LB Agar Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. LB Agar Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation

Specifications
SKU 113002204-CF
Application Notes LB Agar Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. LB Agar Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Cells Tested E. coli
Certifications Molecular Biology Certified™.
Media Type Bacterial Media
Format solution
Formulation Details Typically contains 10 g Tryptone; 5 g Yeast Extract; 10 g NaCl; 15 g Agar-B. Small quantities of biological buffers have been added for proper pH adjustment.
Grade Molecular Biology Certified™ Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.5 ± 0.3 at 35 °C.(Lit.)
Physical Appearance Solution
Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in LB Agar Medium formulations. Chosen reagents are combined in the proper proportions according to the LB Agar formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Presentation Bottle
Product Overview LB-Agar medium, a common bacterial growth medium for E.coli cells, is widely used in molecular biology. The two main components of LB media are Tryptone and Yeast Extract. Tryptone is used in a concentration of 10 gram / liter and Yeast Extract in a concentration of 5 gram / liter. LB medium is the high salt LB medium type with 10 gram /liter. LB Medium is also known as Luria-Bertani Media, or L-Broth Base, Miller Formulation. Agar is included in this convenient formulation.
Recommended Replacement For L-Broth Agar
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology
Sterility Sterile
Storage and Handling Store at 4°C.
Typical Working Concentration 40 g/L
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.