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L-BROTH AGAR (POWDER)

L-BROTH AGAR (POWDER)

Synonyms Luria Broth Base with Agar

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SKU 113001232-CF
Price:
$156.35
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Description

Product Description

L-Broth Agar is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains. MP Biomedicals offers L-Broth Agar medium in an encapsulated format for convenience and ease of use.

Application Notes

L-Broth is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. L-Broth supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation

Specifications
SKU 113001232-CF
Application Notes L-Broth is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. L-Broth supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Cells Tested E. coli
Certifications Molecular Biology Certified™
Clarity NTU Clear once disolved in water
Media Type Bacterial Media
Format powder
Formulation Details Typically contain 10 g Tryptone; 5 g Yeast Extract; 0.5 g NaCl; 15 g Agar. Small quantities of biological buffers have been added for proper pH adjustment.
Grade Molecular Biology Certified™ Bacterial Growth Medium
Applications Cell culture |Proteomics |Purification and Detection |Recombinant Protein Expression and Analysis |Bacterial Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Genetic Manipulation
Kit Compatibility Any Plasmid Purification Kit including MP's RapidPURE™, RPM™, and PhoenIX™ Lines
Personal Protective Equipment Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Acceptable pH range is 6.6 ± 0.3 at 35 °C. Please note that the pH of L-Broth Agar Medium liquid increases as the temperature of the solution decreases.
Physical Appearance Powder
Preparation Method Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in L-Broth Agar Medium formulations. Chosen reagents are combined in the proper proportions according to the L-Broth Agar formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Presentation Jar
Product Overview L-Broth, a common bacterial growth medium for E.coli cells, is widely used in molecular biology. The two main components of LB media are Tryptone and Yeast Extract. Tryptone is used in a concentration of 10 gram / liter and Yeast Extract in a concentration of 5 gram / liter. L-Broth is the low salt (NaCl) LB medium type with 0.5 gram /liter. L-Broth medium capsules have same high quality formulation as L-Broth powder with the added advantage of being in a capsule form, eliminating the need for weighing and handling. The growth characteristics are the same as L-Broth powder. L-Broth agar includes 15 g/L Agar-B in the formulation
Recommended Replacement For CIRCLEGROW® Agar
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Environmental and Ecology
Solubility Soluble in water (31 g/L).
Storage and Handling Store dry L-Broth Agar at ambient temperature (15-30°C). Powder medium is hygroscopic; tightly cap; minimize exposure to air. Store reconstituted media at 4°C.
Typical Working Concentration 31 g/L
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.